PRINCIPAL INVESTIGATORPROGRAM DIRECTOR Sandra Handwerger, M.D 
Table 1. Bioassays for destruction and/or adsorption of 
vancomycin by strain VR, using vancomycin susceptible 
strains of S. sanguis and S. pneumoniae 
S. 
control 
incubation with VR: 
2 hours 
4 hours 
18 hours 
18 hours, "gentle" 
filtration 
18 hours, sheared 
with glass beads 
MIC (ug/ml) 
sanguis 
S. pneumoniae 
Wicky 
R6St 
0.5 
0.25 
0.5 
0.25 
1.0 
0.5 
0.25 
0.25 
0.25 
Table 2. 
Plasmids identified 
in Leuconostoc isolates 
strain 
vancomycin MIC 
plasmids (size in kb) 
(ug/ml) 
688 
1.25 
80, 14, 3.8, 2.8 
39 
2.5 
60, 14, 3.8 
14AM 
1.25 
60, 14, 3.8 
VR 
>512 
none identified 
VR1 
>512 
2.8 
VR2 
>512 
80, 40, 10.5, 8 
8293 
>512 
80 
33313 
>512 
none identified 
Recombinant DNA Research, Volume 13 
[437] 
