27942 
NOTICES 
XI. TRAIN INC AIDS, MATERIALS AND COURSES 
A. Slide-Tape Cassettes 
1. Assessment of Risk In the Cancer Virus 
Laboratory ($10). 
2. Effective Use of The Laminar Flow Bi- 
ological Safety Cabinet ($10) . 
3. Formaldehyde Decontamination of Lam- 
inar now Biological Safety Cabinets ($10) . 
4. Certification of Class II (Laminar now) 
Biological Safety Cabinets ($13). 
5. Hazard Control In the Animal Labora- 
tory ($10). 
6. Basic Principles of Contamination Con- 
trol (In preparation) . 
7. Selection of a Biological Safety Cabinet 
(In preparation) . These slide tape cassettes 
are available for purchase from the National 
Audiovisual Center. The price for each is 
given above after the title. Send your order 
prepaid with a check or money order made 
payable to National Archives Trust Fund and 
mall to: Sales Branch. National Audiovisual 
Center (GSA), Washington, D.C. 20409. 
8. Research Laboratory Safety. This slide 
tape cassette, stock number 176.79, Is avail- 
able for $75 from the National Safety Coun- 
cil, 425 North Michigan Avenue, Chicago, 
Illinois 60611. 
B. Film's 
1. Air Sampling for Microbiological Par- 
ticulates (M-926), 
2. Handling the Laboratory Guinea Pig 
(T2618-X) . 
3. Handling the Laboratory Mouse (T2617- 
X). 
4. Infectious Hazards of Bacteriological 
Techniques (M-382). 
5. Laboratory Design for Microbiological 
Safety (M-1091). 
6. Plastic Isolators: New Tools for Medical 
Research (M-599). 
7. Safe Handling of Laboratory Animals 
(M— 455 ) . 
8. Surface Sampling for Microorganisms 
(Rodac Method) (M-924). 
9. Surface Sampling for Microorganisms 
(Swab Method) (M-925) . 
These films are available on loan without 
charge from: Media Resources Branch, Na- 
tional Medical Audiovisual Center (Annex) , 
Station K, Atlanta, Georgia 30324. 
The same films (except 2 and 3) can be 
rented or bought from: National Audiovis- 
ual Center (GSA) (Rental Branch) = (Sales 
Branch), Washington, D.C. 20409. 
C. Courses 
1. Biohazard and Injury Control in the 
Biomedical Laboratory. Presented by the 
University of Minnesota, School of Public 
Health and the National Cancer Institute, 
Office of Research Safety. Direct inquiries to 
Dr. Donald Vesley, University of Minnesota, 
1 School of Public Health, 1325 Mayo Memo- 
rial Building, Minneapolis, Minnesota 65465. 
June 22-24, 1976, Los Angeles, CA; October 
26-28, 1976, Boston, MA; December 7-9, 1976, 
Bethesda, MD. 
2. Biohazard Containment and Control for 
Recombinant DNA Molecules. Presented by 
the University of Minnesota, School of Pub- 
lic Health and the National Dancer Institute, 
Office of Research Safety. Direct Inquiries as 
above. September 8-9, 1976, Stanford, CA: 
September 21-11, 1976; Cold Spring Harbor, 
NY. 
3. Safety in Laboratory. Presented by Na- 
tional Institute of Occupational Safety and 
Health, Division of Training and Manpower 
Development, by special arrangement. Rob- 
ert A. Taft Laboratories, 4676 Columbia Park- 
way, Cincinnati, Ohio 45226. 
4. Laboratory Safety Management. Present- 
ed by the Laboratory and Training Division, 
Bureau of Laboratories, Center for Disease 
Control, Atlanta, Georgia. September 14-16, 
1976, September 13-16, 1977. 
IS. OUTLINE OP A SAFETT AND OPDIATION 
MANUEL FOR A P4 FACILITY 
A. Purpose 
B. Policy 
C. Responsibility and Authority 
1. Management. 
2. Supervisor. 
3. Each Employee. 
4. Facility Safety Officer. 
5. Biohazard Safety Committee. 
D. Facility Assignment Procedures 
E. Reporting of Major and Minor Accidents 
and Injuries, Exposure to Toxic or In- 
fectious Materials, Unsafe Conditions 
and Property Damages, and Rendering 
First-Aid 
F. General Laboratory Safety 
1. Fire. 
2. Equipment. 
3. Physical. 
4. Chemical. 
5. Radiological. 
G. Safety Procedures Associated with Bio- 
hazard Activities of the Laboratory 
1. Personnel Practices. 
2. Operational Practices. 
H. Medical Surveillance 
I. Facility Operations 
1. Personnel Access Procedures. 
2. Access Procedures for Equipment Mate- 
rials and Supplies. 
3. Maintenance and Support. 
4. Zone Classification. 
5. Facility Monitoring Procedures. 
6. Housekeeping. 
J. Others 
1. Packaging and Shipment of Biohazard- 
ous Materials. 
2. Emergency Procedures. 
3. Insect and Rodent Control. 
4. Orientation and Trai nin g 
Appendix D was prepared by a Working 
Group Consisting of : 
W. Emmett Barkley (Chairman), National 
Cancer Institute, NIH. 
Manuel S. Barbeito, National Cancer Insti- 
tute, NIH. 
Everett Hanel, Jr., Frederick Cancer Research 
Center. 
George S. Michaelsen, School of Public 
Health, University of Minnesota. 
Vinson R. Oviatt, Division of Research Serv- 
ices, NIH. 
Warren V. Powell, Division of Research Serv- 
ices, NIH. 
John Richardson, Center for Disease Control. 
James F. Sullivan, National Animal Disease 
Laboratories. 
Arnold G. Wedum, Frederick Cancer Re- 
search Center. 
REFERENCES 
(1) American National Standards Insti- 
tute. 1968. Z 35.1. 
(2) Wedum, A. G. 1964. Laboratory safety 
in research with infectious aerosols. Public 
Health Rep. 79:619-633. 
(3) Phillips, G. B. 1965. Microbiological 
hazards in the laboratory. J. Chem. Educa- 
tion. Part One — Control, 42:A43-A48. Part 
Two — Prevention, 42:A117-A130. 
(4) Heilman, A. 1969. Biohazard control 
and containment in oncogenic virus re- 
search. USPHS, NIH, NCI. 
(5) Darlow, H. M. 1969. Safety in the 
microbiological laboratory. In J. R. Norris and 
D. W. Ribbons (eds.) . Methods in Microbiol- 
ogy. Academic Press, New York, pp. 169-204. 
(6) Chatigny, M. A. and Clinger, D. I. 1969. 
Contamination control in microbiology. In R. 
L. Dimmick and A. B. Akers (eds.). An In- 
troduction to Experimental Aerobiology .-John 
Wiley & Sons, New York, pp. 194-263. 
(7) Chatigny, M. 1961. Protection against 
infection in the microbiological laboratory, 
devices and procedures. In W. W. Umbreit 
(ed.). Advances in Applied Microbiology No. 
3. Academic Press, New York, pp. 131-192. 
(8) Collins, C. H., Hartley, E. G., and Pils- 
worth, R. 1974. The prevention of laboratory- 
acquired infection. Public Health Laboratory 
Service Monograph Series No. 6. Her Majesty's 
Stationery Office, London. 
(9) Anon. 1968. Good Laboratory Practices 
Manual. National Cancer Institute, Bethesda. 
MD. _ 
(10) UB. Public Health Service. 1974. NIH 
Biohazards Safety Guide. GPO Stock #1740- 
00383. Supt. Documents, U.S. Government 
Printing Office, Washington, D.C. 204C2. 
($3.85) 
(11) Baldwin, C. L„ Lemp, J. F., and Bar- 
beito, M. S. 1975. Biohazards assessment in 
large-scale zonal centrifugation. Appl. Micro- 
biol. 29:484-490. , 
(12) Morris, C. A. and Everall, P. H. 1972. 
Safe disposal of air discharged from centri- 
fuges. J. Clin. Path. 25:742. 
(13) Hall, C. V. 1975. A biological safety 
centrifuge. Health Lab. Sci. 12:104-106. 
(14) Reitman, M. and Wedum, A. G. 1966. 
Microbiological safety. Public Health Rep. 
71:659-665. 
(15) Hanel, E. and Kruse, R. H. 1967. Lab- 
oratory-acquired mycoses. Misc. Publ. 28. AD- 
665376. Frederick Cancer Research Center, 
P.O. Box B, Frederick, MD. 
(16) Smadel, J. E. 1951. The hazard of ac- 
quiring virus and rickettsial diseases in the 
laboratory. Amer. J. Public Health 41:788- 
795. 
(17) Heckly, R. J. Quoted in A. H. Harris 
and M. B. Coleman (eds.). 1963. Laboratory 
infections and accidents. In Diagnostic Pro- 
cedures and Reagents. Amer. Public Health 
Assoc., Inc., New York. 
(18) Grieff, D. 1969. Safe procedure for 
opening evacuated glass ampoules containing 
dried pathogens. Appl. Microbiol. 18:130. 
(19) Harney, R. W. S., Price, T. H„ and 
Joynson, D. H. M. 1976. Observations on en- 
vironmental contamination in a microbio- 
logical laboratory. J. Hyg. Camb. 76:91-96. 
(20) Rutter, D. A. and Evans, C. G. T. 1972. 
Aerosol hazards from some clinical labo- 
ratory apparatus. Brit. Med. J. 1:594-597. 
(21) Godber, G. 1975. Report of the work- 
ing party on the laboratory use of danger- 
ous pathogens. Cmnd 6054, ISBN 0 10 160540, 
40 pp. Her Majesty's Stationery Office. Lon- 
don. 
(22) Idoine, L. S. (ed.) 1973. Centrifuge 
Biohazard Proceeding of a Cancer Research 
Safety Symposium. Frederick Cancer Re- 
search Center, Frederick, MD. 
(23) Hers, J. F. Ph. and Winkler, K. C. 1973. 
Airborne transmission and airborne infection. 
John Wiley & Sons, New York. 
A. Page 415. 
B. Page 425. 
C. Page 429. 
D. Page 434. 
E. Pages 436-437. 
F. Page 461. 
G. Page 494. 
(24) LidweU, O. M. 1967. Take-off of bac- 
teria and viruses, pp. 116-137. In P. H. Gre- 
gory and J. L. Monteith (eds.). 17th Sym- 
posium of the Society for General Microbio- 
logy, Cambridge Univ. Press, London. 
A. Page 117. 
B. Page 123. 
C. Pages 127-128. 
D. Page 130. 
(25) Lennette, E. H., et all 1974. Labora- 
tory Safety regulations, viral and rickettsial 
disease laboratory. California State Depart- 
ment of Health, Berkeley, CA. 
(26) U.S. Public Health Service. 1975. Lab 
safety at the Center for Disease Control. 
DHEW Publication No. CDC 76-8118. USPHS, 
CDC, Atlanta, GA 
(27) U.S. Army. 1969. Safety regulations, 
microbiological, chemical and industrial safe- 
ty. FDR 385-1. Fort Detrick, Frederick, MD. 
(28) Lidwell, O. M„ Brock, B„ Shooter, 
R. A., Cooke, E. M., and TTlomas, G. E. 1975. 
FEDERAL REGISTER, VOL. 41, NO. 131 — WEDNESDAY, JULY 7, 1976 
[ 42 ] 
