/.j\ Materials arc equipment that contain or have come in contact with 
chese organ'! sms nust be disinfected or sterilized by autoclaving prior 
t; disposal , and wo rk surfaces decontaminated, (ii) Cotton plugged 
pipettes may be used where the hazard is minimal, but mechanical 
oisettina devices are preferable and are required for more hazardous 
•Taterial . (iii) Pointed syringe needles should be avoided where possible, 
(i. Eat-' r c, drinking, ard smoking are not permitted in the work area. 
{v Laboratory personnel should wash hands before and a^ter experiments 
involving these "materials, (vi) Laboratory doors must be closed while 
s„c r expe'"' - e r ts are in progress, (vii) Appropriate clothing such as clean 
'aborafc or„* coats, or similar apparel , and closed shoes should be worn 
,^e r handling potentially hazardous organisms (cf.ref.12). Laboratory 
coats should not be worn outside the work area, (viii) Each laboratory 
should hc/e =" effective insect- and rodent-control program, (ix) Any 
neseanc^ group working 'with agents with a known or potential biohazard 
should "a.e an e'C'-gercy plan, including a clean-up procedure to follow if 
a r aoc'ce r t co r ta"i r ates oenscnnel or environment. The principal in- 
/est J gator ~ust ensure that everyone in the laboratory is familiar with 
both the o>cte r tial hazards of the work and the emergency plan, (x) If 
a •'esea'*c 1 ' group is working with a known pathogen for which an effective 
vacc're is available, a' 1 workers should be immunized, (xi) Serological 
"O r 'toring, ^^e^e aopropriate, should be provided for. 
-• z, ~ys~ca' conta-'nrent levels - A variety of combinations (levels) 
c r soec J a‘ procedures, equipment, and laboratory installations that provide 
add^tiona o r ys'ca' barriers can be formed. For example, 31 combinations 
are 'sted ■ n Laboratory Safety at the Center for Disease Control" (4); 
[ 76 ] 
