26 
<a> Shotgun Experiments 
These experiments involve the production of recombinant DNAs 
between the Vector and the total bNA or (preferably) any partially 
purified fraction thereof from the specified cellular source. 
(i ) Eukaryotic DNA recombinants 
Primates - P3 physical containment + an EK3 host-vector, or 
P4 physical containment + an EK2 host-vector, except for DNA from 
embryonic tissue or primary tissue cultures therefrom, and germ- 
line cells for which P3 physical containment + an EK2 host-vector 
can be used. The basis for the lower estimated hazard in the case 
of DNA from the latter tissues is their relative freedom from 
horizontally acquired adventitious viruses. 
Other mammals - P3 physical containment + an EK2 host-vector. 
Birds - P3 physical containment + an EK2 host-vector. 
Cold-blooded vertebrates - P2 physical containment + an EK2 
host-vector except for embryonic or germ-line DNA which require P2 
physical containment + an EK1 host-vector. 
Other cold-blooded animals and lower eukaryotes - P2 physical 
containment + an EK1 host-vector. If the eukaryote in this class is a 
known pathogen (i.e., an agent listed in Class 2 of ref. 5 or a plant 
pathogen) or carries such an agent, the containment should be increased 
to P3 + EK2 . 
Higher plants - P2 physical containment + an EK2 host-vector. 
If the plant carries a known pathogenic agent or makes a product known 
to be dangerous to any species, the containment should be raised to P3 
physical containment + an EK2 host-vector. 
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