87 
Materials which may be damaged by temperature or steam are 
sterilized by gaseous or vapor methods in an air lock de- 
signed for this purpose. 
h) Eating, drinking, smoking, and storage of food are not 
permitted in the facility. Water fountains located in the 
facility corridors are permitted. Separate potable water 
pipes are provided for these fountains. 
i) Facilities to wash hands are available within the facility, 
and personnel are instructed to wash hands after experiments. 
j) An insect and rodent control program is provided. 
k) Animals not related to the experiment are not permitted in 
the facility. 
l) If an appropriate central vacuum system is provided, each 
vacuum outlet is protected by filters and liquid traps. 
m) Use of the hypodermic needle and syringe is avoided when- 
ever possible. 
I want to close on two points. First, I want to underscore again the 
importance of training. Workers in this field must be proficient in micro- 
biological techniques — techniques that will prevent the release of con- 
taminants from the experimental system. They must be proficient in standard 
safety practices. One does not confine a poorly contained study into a hood 
and assume that the hood is going to provide the protection. The technique 
is very important in preventing the release of these materials. 
Second, everyone who occupies laboratory space where DNA recombinant 
studies will be undertaken must be knowledgable of the risks associated 
with this work and the means by which this risk is to be controlled. 
DR. FREDRICKSON: Thank you, Dr. Barkley. 
I think that we will offer this opportunity now for members of the 
committee to question Dr. Barkley or to comment on the material that he has 
just described, if you would. 
Dr. Petersdorf? 
DR. PETERSDORF: Well, I wanted to make a comment which pertains to 
some of this morning's discussion to try to put into perspective some of the 
area of microbiological research with pathogenic organisms. 
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