120 
Okay, those experiments are not listed as experiments to be deferred 
at the present time. This is the slide which I showed you before (9, page 
47) which summarizes the position that the guidelines take on this parti- 
cular aspect — namely, for mammals and warm-blooded animals as a source of 
foreign DNA and introduction into coli. This is divided up rather than 
all being together. The mammals are divided into primates and other mam- 
mals, and then the other warm-blooded animals are the birds, so that we 
are concerned with the comparison between the request on page 3 that such 
experiments not be done and these particular guidelines. 
So for the primates, as I pointed out before, the guidelines recom- 
mend P3 physical containment with an EK3 host-vector system, or P4 physical 
containment and EK2 host-vector systems, except in the case of where the 
sources of foreign DNA are embryonic or germ-line DNA for the reasons I 
outlined before. The advisory committee concluded that such DNA presented 
less of a hazard primarily because the possibility of introducing DNA from 
viruses or other adventitious agents is lowered. 
Other mammals are at P3 and EK2; and birds, which are the remaining 
classification of warm-blooded animals, are also at P3 and EK2. This has 
been summarized for you where the guidelines are as opposed to the point 
made in this document that such experiments be banned. 
Slide 9 
Guidelines for Experiments with E. coli 
“Host- Vector" Systems. I 
'Foreign' DNA = Mixed Fragments = ''Shotgun'' 
Containment 
Foreign DNA Source 
Physical 
Biological 
Eukaryoles: Primates 
P3 
EK3 
P4 
° r EK2 
Embryonic 
P3 
EK2 
Other Mammals 
P3 
EK2 
Birds 
P3 
EK2 
Cold Blooded Vertebrates 
P2 
EK2 
embryonic 
P2 
EK1 
Lower Eukaryotes 
P2 
EK1 
if pathogenic 
P3 
EK2 
Higher Plants 
P2 
EK2 
if pathogenic 
P3 
EK2 
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