121 
Okay, now on page 3, item number 3, it says the use of currently 
available animal viruses as vectors are also not to be performed. I didn't 
put this slide back in the machine, but you will recall that I summarized 
it this morning. We could have the lights now, I think, please. 
Two animal virus vectors are discussed in the guidelines, polyoma 
and simian virus 40, and distinction is made between the containment 
afforded by the two of them. Polyoma is not known to infect humans or 
human cells in tissue culture, therefore the conclusion was made that it 
was unlikely that polyoma virus particles containing recombined DNA 
fragments would escape from laboratories in a meaningful manner, 
because of the inability to infect humans, and therefore polyoma could be 
used as a vector under the specified conditions, which were P3 for any 
non-pa thogenic organism, and P4 for any pathogenic organism in class 2 of 
CDC, with P3 being allowed if no virus particles were being made in the 
experiment, and with the additional proviso that a defective polyoma had 
to be used as the vector, and not an intact wild-type polyoma. 
Similar considerations are for SV40, but with the added proviso that 
because it infects humans and therefore can be carried out of laboratories, 
the physical containment requirements for experiments with DNA derived 
from non-pa thogenic species were at P4, and there were no other experi- 
ments permitted except experiments where no virus particles were made, 
which could be done at P3, or where the DNA was highly purified and shown 
not to contain harmful genes. 
So this then summarizes for you the position the guidelines take on 
this same point, which is number 3 here. 
Okay, another point of disagreement is item number 4 on page 3 — the 
introduction into _E. coli . of the whole genome or any purified segment of 
a viral DNA which is oncogenic in any species. Also, the formation of 
hybrids of total or partial oncogenic virus DNA molecules, with _E. coli 
vectors, whether or not these hybrid molecules are subsequently intention- 
ally cloned in E. coli . 
The guidelines make no distinction between oncogenic viruses, on- 
cogenic animal viruses, and any other animal virus in terms of the intro- 
duction of such — of DNA derived from animal viruses into _E. coli . All 
such animal viruses are treated together. 
If anybody wants to look at this in the guidelines, it is under c(l). 
I haven't got the page, maybe somebody could find it. 
SPEAKER: Page 11. 
DR. SINGER: I have the summary. It is on page 6 of of the summary. 
Okay, it says there that if the foreign DNA is from any virus that infects 
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