150 
Incidentally, anybody who has worked in the tissue culture lab knows 
that there are frequently contaminations of tissue cultures with bacteria, 
and a naturally occurring SV40 recombinant could potentially insert itself 
into E_j_ coli , and unbeknownst to the researcher the experiment we have 
been trying to avoid could well happen. 
The fourth area is inserting tissues from human transmissible diseases 
into animals known to have similar naturally occurring diseases. An example 
of this is human hepatitis. Human hepatitis A virus has been transmitted 
in primates known to have their own form of hepatitis virus. A naturally 
occurring human-animal recombinant hepatitis virus is always a possibility. 
It could well be stable, novel, and uniquely pathogenic. 
Another example is systemic lupis erythematosus, which is at this 
point a candidate virus disease in man. Transmissible agents have been 
demonstrated in certain animal species for SLE, and putting SLE tissue 
into these species could allow for a unique recombinant SLE. 
I think I will stop at that point. I would just recommend that the 
committee consider these area, and also I would urge a very conservative 
attitude on these experiments. Benefits may come in time, but first I 
think we must prove to our own satisfaction that we are going to do no harm. 
DR. FREDRICKSON: Thank you. Dr. Wiesenfeld. We have four minutes 
for any final questions or discussion on Dr. Wiesenfeld comments. If not 
from the committee, I see a hand back there. 
Yes, Mr. Madansky? 
MR. MADANSKY: I would just like to add one other area in which 
possible recombination can take place, which perhaps the committee hadn't 
thought of or hadn't considered. That is that area which would involve 
doing experiments under low containment facilities or none at all, but 
doing unrelated experiments in the same lab. For instance, doing re- 
striction mapping of animal viruses in one corner of the lab, and then 
doing a shotgun of any kind of experiment in cloning on an invertebrate 
in the other corner of the lab. Possibly, and apparently it has been 
known to happen, you can get one piece that shouldn't join into the 
invertebrate DNA, and get cloned. Therefore, you have a situation where 
you really should have had containment facilities much higher because 
two unrelated things go on in the same laboratory. 
DR. FREDRICKSON: Thank you. 
Dr. Barkley, did you have a comment? 
DR. BARKELY: Yes, I asked Dr. Wedum if he would kindly prepare a 
historical review of the approximately 400 and some infections that were 
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