158 
Now, if fact, that is a way of selecting out specif icially or really in 
reaching for it to a reasonable point, a particular DNA with its control 
regions as chopped up by restriction enzymes, and then being put into the 
Bruce Alberts system are reasonable alternatives. 
DR. FREDRICKSON: I think we may be moving slightly afield. 
Thank you. Dr. Sedat. 
I would like to call upon Dr. Goldstein. Dr. Goldstein is from Harvard. 
DR. GOLDSTEIN: Would you like me to do the comparison of these two 
sets of guidelines first? 
DR. FREDRICKSON: I think that would be very helpful. 
DR. GOLDSTEIN: Okay, I am going to try and do a quick summation, and 
not go into all the specific details. The details are there, and everybody 
has copies. 
DR. FREDRICKSON: Let us — Dr. Goldstein, excuse me. Let me then am- 
plify for the committee that you are now going to compare the present 
guidelines with some of the suggestions made by you and others in a docu- 
ment submitted from the Boston DNA Recombinant Group, correct? 
DR. GOLDSTEIN: Yes. 
DR. FREDRICKSON: Thank you. 
DR. GOLDSTEIN: Okay, the first place we differ is the basis for a good 
bit of this work, and that is, E, coli is opposed to as an organisms of 
choice. 
We still feel that it is the wrong choice, because as everyone knows, 
it is a human inhabitant. Right now there is nothing better, and we feel 
if the work is to go on, it should go on in an EK2 EK3 extreme. We also 
feel that the NIH, through the use of contract support and other types of 
encouragement should very strongly encourage a search for a better organism. 
The second place we differ is — well, I will pick an example of it. 
There is, in the La Jolla guidelines, a provision where invertebrates, for 
some reason, are separated from vertebrates and therefore shotgunning exper- 
iments are allowed to be carried out. We feel that the dangers inherent in 
shotgunning invertebrates are just as dangerous as any vertebrate, mainly 
because of the unknown factor, and that unknown factor is what are all those 
genes you are shotgunning? They have never been defined, so you are not 
certain what you are working with. 
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