14 
For instance, in 1959 there was aerosol challenge of 46,412 mice, 3,013 
guinea pigs, 25 rabbits, 276 monkeys, and 2 chimpanzees. Work included 
the etiologic agents of anthrax, coccidioidomycosis, plague, psittacosis, 
Q fever. Rocky Mountain spotted fever, tularemia, Venezuelan encephalitis, 
yellow fever, and two others. 
3. 1960 - 1969, inclusive, with 52 infections 
These 10 years featured the introduction of an effective live 
tularemia vaccine in June 1959, with the result that there was only one 
hospitalized case, in 1961 in a man who had not received the living vaccine, 
although he had a long history of 17 injections of killed vaccine. There 
were 10 mild nonhospital ized tularemic infections; 9 had had the live 
vaccine and 1 with a cutaneous lesion had had pulmonic tularemia in 1957. 
There was 1 hospitalized VEE case in 1961. But after vaccination with live 
VEE vaccine began in 1963 there were only 3 mild cases. These three had 
had the live vaccine. 
There were a few cases of other laboratory infections, but the only 
single agent causing a significant number was Coxiella burneti (Q fever); 
there were 22 cases, of which only 7 were ill enough to require hospitaliza- 
tion. The 22 infections were scattered among 8 buildings, not including 
3 infections of uncertain origin among craftsmen and engineers. 
From the viewpoint of evaluating effectiveness of Class I cabinets, 
the record of Building 560 is noteworthy. This building housed medical 
microbiological units responsible for basic research on (1) tularemia, 
(2) plague, (3) genetics, (4) bacterial nutrition, (5) microphysiology, and 
(6) staphylococcal toxin. 
There were over 100 persons daily at risk in the six units, but most 
of the microbial agents were not highly infectious. Tularemia infections 
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