18 
with gastight cabinets only, and assigned work of major hazard with the most 
infectious and toxic agents. This was basic and applied research using a 
variety of techniques, procedures, equipment, and small animals, with some 
incidental aerosol challenge of animals. At that time, it was the only 
organization with such equipment. The record is as follows: 
1952: 
One cutaneous chronic brucellosis 
1953: 
One nonhospi tal ized subclinical coccidioidomycosis, 
and one glanders 
1954: 
One nonhospital ized subclinical coccidioidomycosis 
1955: 
One tularemia 
1957: 
One hospitalized coccidioidomycosis 
1958: 
One tularemia 
1959: 
This organization moved into a new all -Class III 
Building 1412. 
1959-1969: One nonhospi tal ized cutaneous blastomycosis in 1966 
2. Another separately administered research unit occupied that portion 
of Building 1412 designed for animal aerosol exposure in two stainless steel 
Freon-tight chambers of 18,000 cubic feet and 30,000 cubic feet, and in two 
smaller tanks each of 1,500 cubic feet volume. From December 1959 to 
December 1969, the 55 employees, of whom 45 were daily at risk, carried a 
weekly work load that commonly included the intracerebral inoculation of 
6,000 to 8,000 mice, and the whole-body exposure to microbial aerosols of 
200 to 300 guinea pigs and 20 to 30 monkeys with all the associated preliminary 
and subsequent procedures. Work was with such agents as those causing 
tularemia, plague, brucellosis, anthrax, Venezuelan encephalitis, coccidioido- 
mycosis, botulism, and Q fever. There was only one infection (cutaneous, 
nonhospi tal ized) during the ten years. 
[389] 
