32 
Cakxe — I->rnu'ii Hoi of Citrus. 
Ji! iuff'ctiuo' fniit ’uitli mycelium on media or Avith tissues 
from affe<-ted fruits the folloAving method Avas used. The fruits Avere 
first AA-asheil in water and tlien Avith alcohol. A small cylinder pene- 
tratino- into tlie ra^' Avas tlum remoA'ed Avith a small cork borer Avitb 
tlie rod allow(Ml to remain loose in the tube. AVith mycelium infec- 
tion a fra^'inent of a^ar A\'ith mycelium from a culture aaus thc'u 
placed in the hole and the outer ])ortion of tho cylinder of tissue re- 
placed by means of the rod in the tube. When infecting' from 
aiiotluu- fruit, a cylinder of tissue from the affected fruit made Avith 
a. cork borer slifihtly lar^'cr than that used on the fruit to bC' 
affected was tlien forced into the opening, in this Avay a tiglit 
fit was sei'iireil to comitensa t(' for sliriiikage. In cutting intAi lemons 
tlie rag shouhl not be ])enetrated so as to cause juice, to doAv, as 
this ai.pearc'd to effectually stop infection. With oranges less, 
trouble was exjierienced from this cause. 
The fungus was found to groAV I'eadilv on leaf fragments uf 
Colocasi.s .sji. infection being obtained by placing them after steri- 
lising in corrosive suiilimate and washing aa'cII. in a decoction of 
lumidia in sterile Avater. Attempts to inoculate the Oolocasia leaves 
by placing conidia in a dro]t on the leaf Avithin a large jar faileh. 
.Nttmnjits to indt'ct hun'es of ttichardui (ifricana Kuntli. also f ailed . 
It is e\-iilent from 11u‘ foregoing that while oranges m;i\' be- 
infected from conidia developed on orange leav'es or fruit, and 
hunons from lemon lea\os and fruit, it has not Ikhui deinonstrateo 
definitely that oranges may be infected liy conidia from lemons or 
vic(‘ versa. As no distinction has lu'en uotml betAveen the groAvth of 
mltiires obtained from either lemons or oranges, the' possibility of 
bit>l()gical strains is suggested. 
(Jermination of oospores has not been obsevA'ed. Xo Clilae;- 
y<los]H)res have been recognised. 
SYSTEMATIC POSITION. 
Clii/to}ili( hora liihrnuiii.^ belongs to the Plutsroli group of Koseu- 
bauin (2 .a), Avhich is identical Avith the Fhytophthortt iufrstaus 
group of Pethyridge (21). This group is based upon the presence 
of a m ph i gy non s antheridia. 
Owing to the past confusion Avith Fytliiacystis citro})Jiihora it 
is desirable to point out some of the differences betAveen the t\vo 
species. It may be stated here that it has been pointed out hy 
several Avriters (3 &: 16) that Fythiacystis is closely allied to and 
should probably be merged Avith Fhytophihora . Smith vk ^mi^m. 
tlu‘ authors of Pytliia<‘ysti.'< haAU' agreed (31) on the close affinity,. 
