Jan., 1904.] Infection Experiments with Species of Rusts. 
57 
ADDITIONAL INFECTION EXPERIMENTS WITH 
SPECIES OF RUSTS. 
W. A. KEI.I.KRMAN. 
{Abstract.) 
General report of artificial cultures of Rust stages, both hete- 
roecious and axitcecious .species, continuation of work reported 
the previous 3 ’ear. Over twent}' species were used and inocula- 
tions of a ver}' large number of host-plants were attempted. The 
experiments numbered nearly two hundred. In nine cases posi- 
tive results were obtained — some being repetitions of previous 
successful experiments, others showing connections not previou.sly 
known. 
Of the latter it was shown that the Black Rust (Puccinia 
muhlenbergiae Arth.) on the common Muhlenberg Grass (Muh- 
lenbergia mexicana) was the alternate stage of the Yellow 
Cluster-cup fungus (Aecidium hibisciatum Schw.) on the Marsh 
Mallow ( Hibiscus moscheuto.s). 
Cultures with the Black Rust (Puccinia cirsii-lanceolati Schroet.) 
of the Common Thistle (Carduus lanceolatus L.) resulted in the 
development of aecidia or Yellow Cluster-cup stage (as well as 
the red and black spores) but this has not before been reported in 
this countr}'. The Rust has heretofore been called Piccinia cirsii 
Lasch, but the experiment showed it to be P. cirsii-lanceolati, a 
species described in Europe several j-ears ago b}’ Schroeter. 
In a similar manner it was proven that the \Yestern Sage Rust 
( Puccinia canlicola Tr. & Gall, on Salvia lanceolata) — material 
for cultures received from Kansas in the early spring (sent by 
Mr. E. Bartholomew) — has a hitherto unrecorded aecidial stage 
on the same host plant. 
Cultures demonstrated experimentallj’ for the first time the aut- 
eu-puccinial character of the Rust of Ruellia. That is to say, all 
of the four stages grow on one and the same host. 
Tlie jxaper is published in full in the December number of the 
Journal of Mycolopy. A summary of the successful cultures may 
be brief!}’ stated thus, it being understood that the teleuto-spores 
(lilack or winter spores) were ixsed when sowings were made on 
the host plants, and, in case of Puccinia lateripes, aecidiospores, 
also ; 
Puccinia angustata (on Scirpus atrovirens) — aecidia on Ej'copus 
americanus. 
Puccinia canlicola (on Salvia lanceolata) — aecidia on Salvia lance- 
olata. 
Puccinia caricis-erigerontis (on Carex festucacea) — aecidia on 
Eeptilon canadensis. 
