424 
The Ohio Naturalist. 
[Vol. VI, No. 3, 
were Anilin Safranin and Gentian Violet, Heidenhain’s Iron- 
Alum-llaematoxylin, and Delafield’s Haematoxylin, the latter 
perhaps giving the best results. Care had to be taken with it 
and the Iron-Alum-Hacmatoxylin as the embryo sacs and em- 
bryos stained so deeply that it was difificult to make out the 
details unless a large part of the stain was removed. The stages 
just after fertilization were quite difficult to observe as the pollen 
tube discharges a quantity of material which stains very deeply 
and obscures the embryo sac structures. 
Orientation for sectioning was not difficult as the ovulary 
when cut crosswise gives longitudinal sections of a number of 
ovules. For the older stages only a portion of the ovulary 
could be sectioned on account of its size. 
The cross section of the very young ovulary shows the 
placentae with minute protuberances which represent the incip- 
ient ovules (Fig. 1). The carpel has three placentae, and the 
ovules are delveoped in six rows which are usually double, but 
this is somewhat irregular. The tip of the ovule remains 
straight for only a short time after the appearance of the arche- 
sporial cell (Fig. 6). The cells along the outer margin begin to 
divide more rapidly than those of the inner side. This unequal 
growth causes the ovule to turn, and this process continues until 
the micropyle is brought close to the funiculus. Before the 
megasporocyte has divided and before the integuments have 
grown over the nucellus the ovule has curved half the distance, 
and the normal anatropous condition is practically attained 
when the ovule has reached the megaspore stage (Fig. .5). At 
this time the characteristic beak which develops at the tip of 
the nucellus is already becoming prominent. 
The integuments lengthen greatly forming a long narrow 
micropyle into which the neck-like process of the flask-shaped 
nucellus i>rojects, even to the tip of the integuments. 
The archesporium is as usual a single hypodermal cell that 
terminates the axial row of the nucellus. It can easily be dis- 
tinguished from the surrounding cells by its greater size and 
deeper color due to the denser protoplasmic contents (Fig. 6). 
By a transverse division the archesporial cell gives rise to two 
cells, the megasporocyte and primary parietal cell (Fig. 7). The 
latter continues to divide by both periclinal and anticlinal walls 
thus forming the parietal layer (Fig. 8) which remains persistent 
and with adjoining cells keeps on dividing to form the long beak 
of the nucellus (Fig. 22). 
The megasporocyte is carried down into the tissue quite a 
distance by the development of the parietal layer before any 
division occurs. The division of the megasporocyte is normal, 
giving rise to four equal megaspores (Fig. 10). The potential 
megaspores soon begin to dissolve and the lower or functional 
