March, 1906.] The Cause of Trembles in Cattle. 
479 
water and milk; these were not offered to No. 6. It had eaten 
leaves and branches of about 35 plants. No inflammation was 
found. 
No. 8. A female rabbit, w’eight after death 1383 grams. 
With aseptic precautions A. E. Guenther, Ph. D., Professor of 
Physiology in the University of Nebraska, injected under the 
skin of her back about cubic centimeters of an extract of 
snake-root made by boiling five ounces of the plants in two 
quarts of water until the liquid was reduced to about two 
ounces, after several days kept at boiling temperature again for 
an hour and heated to boiling a third time just before using. I 
was not looking for immediate results but three minutes after the 
injection was made, while I was still holding her on my knee I 
could feel her tremble and in a minute more we could see tremb- 
ling of the loins very plainly. Half an hour later she showed 
less decided trembling, breathing rapid and deeper than before 
the injection, at times a twitching of loins or sides repeated not 
rapidly enough to call trembling but quite unnatural ; also a 
vibration of the flesh over the angle of the lower jaw, the last, 
perhaps, not caused by the poison. She moved about freely and 
ate lettuce and cabbage. The visible effects of the poison 
lessened through the day and the following day seemed entirely 
gone, except the temperature, which gradually approached the 
normal. The injection was made at 12:15 P. M., Dec. 31. 
11:30 A. M.— temperature 104.8°; 1:30 P. M.— 104.2°; 3:30 
P. M.— 105.1°; 5:30 P. M.— 106.°; 7:30 P. M.— 105.8°; 9:.30 
P. M. — 106.°. Although taken fourteen times between the 
afternoon of Dec. 29th and the time of injection it was in no 
instance above 104.8°. Forty-eight hours after the injection 
the tempertaure had become normal. 
Jan. 3, 7:50 A. M., I began feeding her white snake-root of 
which she took the leaves and branches readily. I kept her 
pretty well supplied each day with snake-root, giving also some 
good food. The effects on her actions and appearance were not 
striking and might have escaped notice if I had not looked for 
them. Deep breathing, sometimes rapid, especially noticeable 
in the loins, with some diminution of strength were noticed. 
She would move about without urging but less rapidlv than a 
normal rabbit. Her temperature taken several times each day 
showed no marked influence of the poison. On the whole it was 
below normal, exceeding 103.8° only on the day she began to eat 
the weed, i. e., before it was digested, and about 23 hours before 
she died, when it was 104. .3°. Jan. 7, her appetite was not so 
good and her eyes dull. In the evening deep breathing was 
noticeable and trembling resembling shivering. Next morning 
I found her lying on her side, as if dead. When laid on top of 
box she gasped for breath, not violently but with increasing 
