280 
The Ohio Naturalist. 
[Vol. VIII, No. 5, 
granule is still clearly visible showing in addition to a few short 
rays stretching away from the nucleus others connecting with 
the nuclear membrane. 
The various irregularities in the eentrosomes are to be com¬ 
pared in the judgment of the writer, to those present in the for¬ 
mation of eentrosomes de novo, under more or less abnormal 
chemical stimulation, in animals. In many of these cases there 
are formed a multitude of small asters two of which grow large 
and form the amphiaster. Because of the difficulty of deter¬ 
mining the sequence of events, having no other indications of 
the relative ages beside the condition of the asters themselves, 
it cannot be asserted that the different asters coalesce or that one 
of them gains the mastery while the other disintegrates. Never¬ 
theless it is to be noted that without exception those asters which 
are interpreted as the end stages were, so far as seen, uniformly 
single (figs. 12-14). 
The activities of the eentrosomes and of the chromatin in the 
reconstruction of the nucleus are apparently independent of each 
other to a considerable degree so that it seems necessary to con¬ 
sider them separately. In the telophase the four chromosomes 
lie loosely in the cytoplasm making a figure not unlike the typical 
daughter star. They have a manifest tendency to converge to 
the centrosome which is more clearly shown in cases where there 
are two asters, when part of the chromosomes may follow the rays 
of each. At the distal ends of the chromosomes, with respect ot 
the aster, there soon appears, in connection with one or more of 
them (fig. 2) a thickening which enlarges at the expense of the 
chromosomes till it becomes the karvosome (nucleolus) of the 
resting nucleus (fig. 2). The transfer of the chromatin to the 
karvosome consists apparently, not so much in the direct absorp¬ 
tion of the chromosomes as in the gradual removal of the chro¬ 
matin from the linin matrix so that in many cases the ends of the 
chromosomes farthest away from the karyosome become vacuo¬ 
late, as it were, and lose their staining reaction giving a gradual 
transition from one condition to the other thereby showing ap¬ 
parently that the chromatin migrates granule by granule from 
the linin matrix (fig. 2.) Those chromosomes which do not di¬ 
rectly connect with the growing karyosome form linin bridges 
across to it, by which the chromatin may be transferred. 
In many cases the nuclear membrane is formed before this 
process is complete and there results a spirem which closely re¬ 
sembles that usually found in the prophase of dividing cells, 
(figs. 7-11). In such spirems all resemblance to the original 
chromosomes mav be lost and a loose, few-meshed network 
formed. In as much as the conduct of the chromatin and of the 
asters are independent, some doubt is thrown on the exact se¬ 
quence of the transformations of the nucleus as well as of the cen- 
