412 
The Ohio Naturalist. 
[Vol. IX, No. 2, 
record to the contrary with reference to the eggs of Asterias 
Forbesii. With the exception of the stronger solution of 
strychnine, Phaseolus was able to live in concentrations which 
would prove fatal to animals. 
The effect of the action of these alkaloids upon plants varies 
both with the nature and with the strength of the solution, but on 
the whole a general rise and intensification of the vital processes 
is seen as, for instance, in the augmentation of the function of 
transpiration, and in the higher rate of regeneration as compared 
with plants not subjected to the influence of stimulating agents. 
It seems therefore, legitimate to assume that there exists an 
intimate relation between the rate of regeneration and the 
physiological condition of the regenerating organism. 
Concerning the rate of regeneration after consecutive opera¬ 
tion it is obvious, from the facts cited above, that after the second 
operation there is a considerable decrease of the power of regen¬ 
eration and, consequently, a slowing down of the process, as well 
as a considerable lengthening of the period which intervenes 
between the operation and the first appearance of regenerated 
tissue. 
The work here recorded was carried on in the Botanical Lab- 
oratorv of the Ohio State University during the Spring of 1908, 
with the aid of a grant from the McMillin Research Fund. 
It gives me pleasure to express here my gratitude to Dr. A. 
Dachnowski for much friendly assistance in this work. 
Cambridge, Mass., October, 190N. 
LITERATURE. 
1. Dachnowski, A.—The toxic property of bog water and 
bog soil. Bot. Gaz. 46: 130-144, 1908. 
2. Goebel, W. — Regeneration in Plants. Bull. Torrey Bot. 
Club. 30: 197-205, 1903. 
3. Livingston, B. E.—Relation of transpiration to growth 
in wheat. Bot. Gaz. 40: 178, 1905. 
4. McCallum, W. B.—Regeneration in Plants. Bot. Gaz. 
40: 241, 1905. 
5a. Morgulis, S.—Observations and experiments on regen¬ 
eration in Lumbriculus. Journ. Exp. Zool. 4: 549-574, 1907. 
5b. The effect of alkaloids on the early development of 
Toxopnenstes variegatus. Proc. Amer. Acad. Arts and Sciences, 
Vol. 44, No. 5, pp. 131-146, 1908. 
6. Pfeffer, W.—Physiology of Plants, Vol. II. 
7. Yasuda, A.—On the effect of alkaloids upon some moulds. 
The Bot. Mag., Tokyo, 15: 79-83, 1901. 
8. Zeleny, C. — The effect of degree of injury, successive 
injury and functional activity upon regeneration in the Scypho- 
zoon Cassiopea xamachana. Journ. Exp. Zool. 5: 265-274, 1907. 
