ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 
97 
cent, solution of carbolic acid to 2 ccm. bouillon, and then 5-15 drops 
of the water to be examined ; the tube is then covered with a rubber cap 
and kept at a temperature of 42°. As soon as the bouillon becomes 
cloudy (8-12 hours) a fresh inoculation in pure bouillon is made from 
this cultivation. This second cultivation will usually be found sufficient 
to get rid of other bacteria, leaving a pure cultivation of immobile 
short diplo-bacilli. If the second cultivation be still impure, then a 
third or fourth may be tried. 
In endeavouring to isolate the bacillus of typhoid from water by 
passing it through three series of cultivations in carbolized bouillon, he 
finds * that this procedure allows another microbe, Bacterium, culi com- 
mune, to develope. Consequently a mixed cultivation may result, giving 
on potato a prominent yellow cultivation instead of a moist colourless 
track, indicative of the simple presence of the typhoid bacillus. It is 
necessary, therefore, to make a plate-cultivation from the carbolized 
bouillon, in order to separate the bacillus of Eberth from its satellite, 
Bacterium coli commune. 
Holst's Bacteriology for Students and Practitioners.f — From the 
fact that this work on bacteriology has been translated from the original 
Norwegian into German, and seems to have been well received, it would 
appear that the aim of the author, which is to deal with bacteriology 
from a practical and non-scientific point of view, has been fulfilled. To 
state the facts of bacteriology in a way to be easily grasped by the student 
and easily aj)plied by the struggling practitioner, is a most desirable 
intention, and one which deserves proper recognition if successful. 
The get-up of the book is on a par with its contents. 
Pocket-book for Students of Bacteriology.:]: — The second edition of 
Dr. H. Bernheim’s pocket manual of bacteriological formulae, useful 
alike to commencing and advanced students, has been enlarged. The 
present edition contains the Soyka-Kral method of making permanent 
cultivation preparations. 
Diagrams for Bacteriological Lectures. § — Dr. C. J. Eberth is now 
publishing a series of diagrams intended for lectures on bacteriology. 
The diagrams, of which three have already appeared (1, Streptococcus 
pyogenes; 2, Cholera bacillus; 3, Tubercle bacilli), are coloured plastic 
representations on a white ground of the micro-organisms copied from 
actual specimens. 
The plates are mounted on canvas, and the magnifications vary from 
20,000 to 60,000, so that the configurations of the bacteria are easily 
visible at a distance of 10-12 yards. 
* Annates de Micrograpkie, ii. (1890) pp. 432-3. 
t Basel, 1891. See Centralbl. f. Bakteriol. u. Parasitenk., x. (1891) pp. 219-20. 
% Wurzburg, 1891. See Centralbl. f. Bakteriol. u. Parasitenk., x. (1891) p. 234. 
§ Berlin, 1891. See Centralbl. f. Bakteriol. u. Parasitenk., x. (1891) pp. 251-2. 
1892. 
H 
