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state clearly that it has disadvantages of a serious kind when the objects 
in view are not thoso which I have tried to explain, and therefore I 
do not offer this new method as anything more than a help to those 
who may try to solve some of the questions to which I have referred. 
Fig. 28. 
Diagrammatic representation of eleven interlamellar cultivations in a moist 
chamber, showing the disposition which I have adopted both for this method and 
other forms of film or plate cultivations. A, Outer basin, containing a thin layer of 
water (W) at the bottom. B, Covering basin, with a flat bottom, allowing a series 
of moist chambers to be piled one above the other in the incubator. C, Inner bell 
preventing condensed water falling upon the slide. D, Plates supported by pieces 
of cork. E’s, Slides. 
On some future occasion I hope to be able to give further details 
regarding the modifications which have been suggested to me by cir- 
cumstances and the general nature of the results I have obtained.” 
Cultivation of Bacilli of Asiatic Cholera.* — Dr. Hueppe cultivated 
cholera bacilli in egg albumen, and by this method so virulent did the 
bacilli become that an injection of the culture into a guinea-pig produced 
toxic results in a few hours, and in a few days toxines were developed 
in sufficient quantities to kill the animal, while formerly forty days 
frequently elapsed before the death of the creature, even if it died at all. 
The active agent was globulin, which developed only in albumen and 
in no other substance. This toxin caused nephritis in most cases. 
The author believes the toxin of cholera to be a pepton. The aerobic 
bacilli of cholera were very resistant to hydrochloric acid, and thus 
adapted to pass through the stomach into the intestine. 
Glass Cover-tube as Substitute for Cotton-wool Plug.f — As sub- 
stitute for cotton-wool, and thereby avoiding many of the inconveniences 
of this system of plugging test-tubes, Dr. Schill advocates a simple 
device which he has adopted for two and a half years with satisfactory 
results. In principle it consists in covering one test-tube with another. 
The cover-tube is about two-fifths the length of the cultivation-tube, 
and both are made quite straight, and not lipped, so that they are easily 
slipped the one over the other, the interspace being about as thick as a 
* International Congress of Hygiene and Demography, 1891, See Lancet, ii. 
(1891) p. 376. 
t Cuntralbl. f. Bakteriol. u. Parasitenk., x. (1891) pp. 657-9 (1 fig.). 
