ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 
159 
axis-cylinder, amyloid degeneration, nucleus, &c. Stains in 1/2 to 1 
minute. 
Gentian violet: — Filtered 3 per cent, anilin solution in water; 
concentrated geutian violet solution in alcohol ; or gentian violet, 
2 • 00 ; ammonia, 0*5; absolute alcohol, 10. For bacteria, &c. At 
ordinary temperature stains in about 21 hours ; 1 hour at 50° C. 
Treat objects with 30 per cent, hydrochloric acid, dehydrate in absolute 
alcohol, clear in oil of cloves, mount in balsam. 
Iodine green : — Iodine green, 0 • 1 part ; water or alcohol, 35 parts. 
Stains in a moment. Mount in balsam. 
Fuchsin (rosanilin): — Fuchsin, O' 25 grin. ; alcohol, 20 ccm. ; water, 
20 ccm. For nucleus, protoplasm, axis-cylinder, elastic tissue, retina, 
&c. ; after staining, treat with alcohol. 
Fuchsin (acid) : — Concentrated solution in water. For nervous 
system. Sections hardened in chromic salts. Keep in stain one hour, 
wash with water, put into alcoholic solution of potash (potash, 1 
grm. ; alcohol, 100 ccm. ; filter after 24 hours ; use 10 ccm. diluted 
with 100 ccm of alcohol) ; wash in water, dehydrate in alcohol 
(saturated with salt), mount in balsam. 
Methylinblue : — (1) Erlich’s concentrated water solution. (2) Koch’s 
concentrated alcoholic solution : methyl blue, 10 ; caustic potash (10 
per cent. 0 • 2 ; water 200). 1 and 2 for bacteria, cover-glass prepara- 
tions; stains in 1/2 to 24 hours; wash in water, dry, mount in balsam. 
For tubercle bacillus ; after staining in blue, transfer cover to concen- 
trated solution of vesuvin (15 minutes), wash well in water, dehydrate 
in alcohol, clear in oil of cloves (micrococcus brown, bacillus blue.) 
Methyl violet: — Methyl violet concentrated alcoholic solution, 11 
ccm.; absolute alcohol, 10 ccm.; anilin water, 100 ccm. For bacteria, 
&c. ; cover-glass preparations ; stains in 24 hours ; put cover for a few 
seconds in nitric acid and 3 parts water ; wash with alcohol ; stain 
with diluted vesuvin solution for a few minutes, wash in 60 per cent, 
alcohol, dehydrate in absolute alcohol, clear in oil of cedar, mount in 
balsam. 
Methyl green: Water solution, 24 per cent. Nucleus, nerves, amy- 
loid substance (degenerated tissue violet, normal green.) Stain in 24 
hours. 
Safranin : — (1) Safranin, 1 part; absolute alcohol, 100 parts; water, 
200 parts. (2) Water, 1 part; alcohol, lpart; safranin, as much as 
will dissolve. For nucleus; washed section stains in a few minutes; 
wash and dehydrate in absolute alcohol, mount in dammar or balsam. 
Water solution (1-1200) for bone development (bone, connective 
tissue, red ; cartilage, yellow). Wash with water slightly acid with 
acetic acid. 
( 5) Mounting', including Slides, Preservative Fluids, &c- 
Balsam Mounting.* — Miss V. A. Latham writes : — “ In reading 
through the January number of ‘ The Microscope ’ I noticed the article on 
mounting. Cajeput oil is not new to me, as I have used it for several 
years, also oil of bergamot and several other oils ; the last which I am 
now working on is terpenol or terbenol (Merck). The reason I first 
* The Microscope, xi. (1891) pp. 281-3. 
