12 
The Ohio Naturalist. 
[Vol. XIII, No. 1, 
observed in Hemerocallis by Fulmer (4). In some cases the 
cytoplasm between two newly formed nuclei was separated 
although no visible wall was formed; but in most cases the nuclei 
were formed and remained imbedded in the general cytoplasm 
(Figs. 28-34). In one case a regular tripartite arrangement of 
three nuclei was observed and these were surrounded by separate 
masses of cytoplasm (Fig. 31). In none of these cases was the 
original sporocyte wall disintegrated. The further development 
of the smaller nuclei was not studied although that might be an 
interesting investigation. 
SUMMARY. 
1. In the reduction division of Fuchsia speciosa there is 
apparently an end to end fusion of the univalent chromosomes, 
forming a continuous spirem which twists and folds up into a 
definite number of loops which represent the incipient bivalent 
.chromosomes, fourteen in number. 
2. The loose network of the resting nucleus at an early stage 
begins to show a massing of chromatin material into indefinite 
lumps of approximately the reduced number of chromosomes. 
These masses probably represent the arrangement of the chromatin 
into a definite mosaic, preparatory to the synaptic conjugation of 
the univalent into the bivalent chromosomes. 
3. Gradually the lumps disappear and the material seems to 
go toward the formation of prominent granules that arrange 
themselves along a delicate thread. 
4. Next follows a period during which there is an evident 
swelling of the nucleus. In consequence of this swelling the threads 
are pulled loose or withdrawn from the nuclear wall, and the chroma¬ 
tin material collapses in a mass. It may collapse around the nucleolus 
or to one side of it, or it may collapse so that the nucleolus has no 
connection with it. The contracted portion may lie in the center 
of the nucleus or in contact with the nuclear wall. This synizesis 
is regarded as an artifact although no definite evidence was 
obtained for or against this supposition. 
5. After the synizesis the spirem is apparently continuous and 
the granules appear small and evenly distributed throughout its 
length. At first there is little or no looping but soon the spirem 
begins to show that it is laid in delicate little loops. The loops 
are arranged on the inside of the nuclear wall. In some sporo- 
cytes as high as eight loops could be determined at a rather early 
stage, still more or less connected, but by the time the fourteen 
loops are fully formed they are usually broken apart. 
6. After breaking apart the loops thicken and tighten until 
masses of various sizes and shapes were formed, four being quite 
small and five of rather large size. 
