260 
The Ohio Naturalist. 
[Vol. II, No. 6, 
significant. 34.2 per cent, of the smut balls were either on or 
within six inches of the wound but within the next six inches 
above and below the wound only 13.6 per cent were to be 
found. 
Row No. 2. 
Each stalk (842 in all) was mutilated as in No. r and then 
smut spores immediately brushed on or painted over the wound. 
The smut used had been kept dry in a tight box since the fall 
of 1900. The work was done after 5 p. m. and was followed by 
a good dew but no rain soon. 
Results: 1 1 .5 per cent, of these stalks were smutted against 
but 8.5 per cent, in the check. The relative position of the smut 
in this part of the experiment was, however, quite significant. 
59.8 per cent, of the diseased stalks were infected within six 
inches or upon the wound, while, in the next six inches above 
and below the wound, only 17.5 per cent, of the bolls were to be 
found. 
Row No. j. 
Each of these 806 stalks was painted at some place with spores 
as in No. 2 but none were mutilated. This was done in early 
evening and although followed by no dew, a fine mist fell the 
next morning. 
Results: 10.4 per cent, of the stalks were smutted against a 
check of 8.6 per cent. Also the percentage of smutted ears to 
total diseased stalk was again significant ; in the check-row 20.9 
per cent, while in the infected row it was but 16.6 per cent. 
Row No. /. 
This row was detasseled during the partly cloudy forenoon of 
August 3. 
Results : 11.5 per cent, of the stalks were diseased against a 
check of 9.5 per cent. The percentage of the total diseased 
stalks having the ear as the affected part was 22.3 per cent, in 
this row and 28.6 per cent, in the check- row. 
.Stated very briefly the results are in accord with those obtained 
at the Indiana and Illinois Experiment Stations in recent years. 
At the stage of growth when the tassels are just appearing, detas- 
seling, mutilation of the stalks lower down, and the application 
of spores without wounding the stalk all cause an increase of 
smut ; and mutilation and the application of spores to the wound 
thus made results in a still larger per cent, of smut. 
