MATURATION AND FECUNDATION OF THE OVUM. 125 
(2) The surrounding protoplasm, which has become scarcely 
distinguishable from the vitellus. 
Each of the chromatic discs gives off a filament, formed, 
during the division of the disc, at the expulsion of the first 
polar globule. Similar filaments appear at the internal ends 
of the discs. The two discs next divide up into smaller 
chromatic globules and the filaments become more numerous — 
in what way is not clear. Thus a spindle is formed. The 
deuterohyalosoma increases in size. 
The spindle is directed radially, one of its poles being deep 
the other superficial. At each of these two poles, outside the 
deuterohyalosoma, appears a star, consisting of a group of 
achromatic granules surrounded by radiating moniliform 
threads. These stars may be — at least in part — formed from 
the substance of the achromatic fibrils of the first figure, but 
the continuity cannot be traced. The rays of the stars directed 
towards the deuterohyalosoma elongate and meet, while the re- 
mainder disappear to a great extent. Thus a figure in the form 
of a lozenge is produced, one of whose diagonals is occupied by 
the spindle within the deuterohyalosoma (see fig. 6, PI. X). 
One half of the lozenge now gradually disappears, and thus 
a figure is formed similar to the ypsiliform figure of the earlier 
period. The deep pole of the axial spindle now approaches 
the surface of the vitellus, and the division of the deuterohyalo- 
soma takes place in a plane passing through the axis of the 
spindle, not in the equatorial plane. 
The second pseudokaryokinetic figure, as Van Beneden calls 
it, is, in its later stages, complicated. An examination of the 
Plate XVIII bis, and of the text referring to it, shows that 
the division of the deuterohyalosoma into two parts — one of 
which is to form the second polar globule, the other the female 
pronucleus — that this division takes place long before the 
second polar globule is expelled from the vitellus. Before the 
change in the position of the spindle occurs, the spindle and 
the deuterohyalosoma are divided axially by a median plate, 
which moves through an angle so as to become superficial, and 
thus the globule is expelled (see figs. 7 and 8, PI. X). 
