300 
SIDNEY F. HARMEE. 
captivity is sufficient to account for the refusal of the larvae to 
attach themselves. 
My observations on the metamorphosis of L. Leptoclini 
are exceedingly incomplete, but they have been sufficiently 
successful to demonstrate that the free larva may produce a 
pair of buds (fig. 55, b), although I am unable to say whether 
the budding takes place normally during a free life, or as a 
general rule only after fixation. In the most successful expe- 
riment, four larvae were kept alive and apparently healthy for 
four days after the commencement of their free existence. 
They had produced buds considerably older than those repre- 
sented in fig. 55, although, as in the adult, they were unequally 
developed on the two sides of the body. The older one of each 
larva was more elongated than those of the figure, and was 
somewhat pear shaped, being attached by its narrow end. 
At its free end was an elongated slit, the opening into the 
vestibule, and these, the oldest larval buds observed, had exactly 
the appearance of those normally developed on the adults soon 
after the opening into the vestibule is a longitudinal slit on the 
anterior side. As I was not aware that I should fail in rearing 
larvae a second time to the same age, I neglected to kill any of 
the four-day larvae, which twenty-four hours later were found 
dead and disorganised. There could not, however, be the 
slightest doubt that the two structures described above were 
really correctly identified, and the larva of L. Leptoclini 
at any rate may be stated to produce a pair of buds in a position 
corresponding to that of the budding regions of the adult. 
The probability that this would be found to be the case had 
already been indicated by Hatschek, who made this suggestion 
on the hypothesis that the dorsal organ was the budding 
region. In my four-day larvae, however, this structure showed 
not the slightest change from its condition at the commence- 
ment of the free existence ; the position of the head was still 
continually varied for convenience of vision (this region of the 
body being exceedingly moveable), and the cilia in connection 
with the dorsal organ, like those of the epistome and ciliary 
ring, were working vigorously. 
