CHROMATOLOGY OF BLOOD OF SOME INVERTEBRATES. 477 
in cvanosulphaem, and perhaps in Stokes’ reduced haematin.” 
Now, Krukenberg 1 tries to show that this reduction is not such, 
hut that chlorocruorin and erythrocruorin are one and the 
same substance which is not reduced by sulphide of ammonium. 
If Krukenberg had studied Professor Lankester’ s paper he would 
have seen that Professor Lankester says that “ on addition 
of reducing agents the two bands are changed into one, having 
nearly the same position as the darker of the two hut fainter. 
On agitation with air the two returned.” The extra- 
ordinary mistake of Krukenberg in missing the fact that 
what he calls “ Ilelicorubin ” — and takes to himself the credit 
of having discovered, although previously discovered by Dr. 
Sorby — is capable of existing in the oxidised and reduced 
state, is an exact parallel to this. 2 
His other criticisms on the action of cyanide of potassium 
on chlorocruorin are based on a mere comparison of spectrum 
maps and are therefore valueless. 
Professor Lankester could not obtain derivatives of chloro- 
cruorin, owing, as he has stated, to the apparent instability of 
this body, which decomposes rapidly. 
I have really nothing of importance to add to the descrip- 
tion given by Professor Lankester, but I have examined the 
aqueous solution of chlorocruorin, its behaviour with some 
reagents, and measured its bands in wave lengths. For the 
sake of comparison I have mapped these spectra in the accom- 
panying Chart I. I have only been able to obtain about a 
dozen specimens, so that my examination is not complete, but 
I hope to be able to study this subject again. 
On slitting up a worm and collecting the green fluid which 
exudes on a watch-glass and examining with the microspec- 
troscope a dark band is seen before D, and a feeble one 
between D and E (sp. 1, Chart I). The brown gills of the 
1 Loc. cit. and ‘ Grundziige einer Yerlgleichenden Physiologie der Farb- 
stoffe und der Farben,’ 1884. 
2 I have shown that this gives the bands of hsemochromogen. Kruken- 
berg’s map of it is quite incorrect. See my sketch in ‘ Proc. Roy. Soc.,’ No. 
226, 1883. I have lately proposed the name “ enterohsematin ” for this 
pigment. 
