CHROMATOLOGY OF BLOOD OF SOME INVERTEBRATES. 479 
was obtained, which, on adding absolute alcohol, showed a hand 
in green, bat as it is very doubtful whether this is not due to 
the action of the acid on a proteid I have not mapped the 
spectrum. 
Hence none of the decomposition products of haemoglobin or 
haematin could be obtained, 1 the pigment, as Prof. Lankester 
had already shown, being destroyed by the reagents required 
to produce acid haematin and hsematoporphyrin. I digested the 
gills of several specimens of Sabella in chloroform, but failed 
to obtain a coloured solution ; digestion with rectified spirit and 
caustic potash furnished a yellow solution, but in this no 
well-marked bands could be detected. 
The blood 2 of the pseudo-haemal system of Serpula con- 
tortuplicata presents some resemblance to that of Sabella, 
and I believe it has not been examined until now. There are 
slight differences in the blood spectra of some specimens, which 
doubtless are due to the pigment being present in different 
states of oxidation, and on comparing some of these spectra 
with those of the histohaematins and with decomposition pro- 
ducts of haemoglobin a striking likeness is apparent. 
On putting a Serpula into the compressorium, and 
bringing gentle pressure to bear on the upper surface of the 
animal, and examining with the microspectroscope, using a 
good achromatic substage condenser, a series of spectra are 
obtained when the various parts of the animal are moved 
under the objective ; what these parts are is seen by looking 
down the left-hand tube of the microscope. 
In this way we can differentiate the blood-vessels, intestine, 
gills, operculum, and other parts, and study the spectrum of 
each. A portion of the pseudo-haemal system, with its con- 
tained blood of a worm gave sp. 7, Chart I, the band before D is 
like that of chlorocruorin, but the first after D and also the 
1 At the same time this colouring matter is certainly closely related to 
hsematin, as sps. 2 and 8 show. In 8 (from serpula-blood) bands like those of 
hsemochromogen are present. 
2 I have not given this pigment a name, as I believe it to be a kind of 
chlorocruorin. 
