480 
C. A. MAC MUNN. 
second are different. In some of the blood-vessels only the 
band before D was visible. 
An aqueous solution obtained from nine Serpulse was a 
reddish-yellow colour by gaslight, yellow by daylight, and this 
gave a spectrum like 7. The band before D was from A 620 5 
to A 593, the second about A 583'5 to A 572, the third uncer- 
tain (about A 551 to A 532). After adding sulphide of ammo- 
nium the only band seen with certainty was that before D. 
which seemed slightly nearer violet. In rectified spirit ex- 
tracts only a faint lutein band was visible in the yellow solution 
from about A 501 to A 477. 
In a specimen in which the blood appeared a bright carmine- 
red colour sp. 8, Chart I, was obtained ; the second band of 
this spectrum resembles the first band of haemochromogen, and 
is really the same as sp. 2. The principal blood-vessel showed 
two round dilatations, and in these I observed sp. 9, Chart I. 
The darkness of the second band at once distinguishes the 
pigment from chlorocruorin. 
In other specimens the same dilated part of the pseudo- 
haemal vessels gave sp. 10, Chart I, while in another specimen 
the blood showed sp. 11, Chart I. 
An aqueous solution of blood obtained from a dozen speci- 
mens — whose blood gave the above spectra — was yellow, and 
showed the three bands represented in sp. 12, Chart I, and 
these gave the following readings : — First band A 618 to A 593, 1 
second A 582 to A 570‘5, third A 551 to A 529'5 (?). On treat- 
ment with sulphide of ammonium the solution became slightly 
greener ; no bands could then be seen after D, and that before 
it was very faint. Hence it would appear that the two- or three- 
banded spectrum denotes the oxidised state. 
On digesting the bodies of some Se rpulae in caustic potash 
and rectified spirit a yellow solution was obtained, giving no 
definable bands, except some feeble shading at the blue end of 
green, but on adding sulphide of ammonium the solution 
became faintly red, and a band like the first band of 
1 I.e., exactly the reading of the first band of oxychlorocruorin. 
