CHROMATOLOGY OP BLOOD OF SOME INVERTEBRATES. 485 
positions : 1st, X 557 to X 545'5, x 2nd, X 524 - 5 to X 501, 3rd, 
X 4945 to X 475. This third band is merged into the second. 
On adding sulphide of ammonium two new bands appear of 
which the 1st is from X 531 to X 507, and 2nd, X 494 - 5 to X 
475, the colour of this solution being changed to yellow and on 
shaking with air remaining the same. With caustic soda 
similar hands appeared and the solution became yellow ; the 
bands read : first, X 532 to X 509 ; and second, X 494 - 5 to X 
477. On neutralising this solution with acetic acid it became 
again faintly red, and the original bands reappeared but were 
very faint. 
On treatment of an absolute alcohol solution with acetic acid 
the colour changed to reddish yellow and sp. 7, Chart II, was 
seen. On treating with caustic soda to alkalinity, the same 
bands as those seen when an alcohol solution is treated with 
that reagent appeared. The spectrum of the original absolute 
alcohol solution is that of the neutral pigment, as can be 
proved. Peroxide of hydrogen did not affect the bands. Hydro- 
chloric acid produced the same effect as acetic acid ; the bands 
reading : first, X 545'5 to X 529’5 ; second, X 51 1*5 to X 488. 
When the alcohol solution is treated with stannous chloride 
the colour changes to yellow, and two very well-marked bands 
appear (sp. 8, Chart II). Dark part of first band X 535 to 
X 51T5; second, X 496 - 5 to X 477. Hyposulphite of sodium 
changed the colour to yellow but the original bands could 
be seen, although faint. 
On evaporating the alcohol solution on the water-bath a 
brownish- red residue was left, in which were numerous crystals 
of chloride of sodium ; on treating with chloroform a fine red 
solution was obtained, but a good deal of the residue remained 
undissolved, and what did remain of it was more of a pink 
colour than its previous colour brown ; 2 on filtering the chloro- 
form the paper was stained a pale rose colour. On a white 
dish this chloroform solution had a violet tinge and gave sp. 9, 
1 If the feeble shading at each side of the band is included it reads from 
X 560 to X 513. 
s Owing to decomposition. 
i i 
VOL. XXV. 
NEW SER. 
