486 
C. A. MAC HUNN. 
II ; on evaporation of the chloroform a brown-red residue with 
a violet tinge was left. This residue was now only partially 
soluble in absolute alcohol, forming a pale reddish-violet solu- 
tion, giving, however, the same spectrum as the original abso- 
lute alcohol solution, and with caustic soda it gave the same 
reaction as before. From the fact, however, that the whole 
of the residue was not now soluble in absolute alcohol, it is 
quite evident that the pigment was decomposed by evaporating 
it down by the aid of heat. I stated above that the residue 
left after the evaporation of an alcohol solution was not quite 
soluble in chloroform, and what was left after the chloroform 
extraction was treated with ether ; this solvent took up a pig- 
ment whose absorption spectrum is remarkable for the two 
narrow bauds in green, which recall to mind some histohaematin 
spectra. This ether solution was reddish, and gave sp. 10, 
Chart II ; its bands reading as follows : first band, X 554'5 to 
X 547 ; second, X 540 to X 535 (?) ; and third, about X 516 to 
484’5 (= darkest part). Although so different in spectrum 
from the alcohol solution, yet when the ether was evaporated 
and the residue again dissolved in absolute alcohol, a rose- 
coloured solution was obtained whose spectrum closely resem- 
bled that of the original alcohol solution, and with caustic 
soda it changed to yellow, and the bauds already referred to 
were seen. Hence the pigment present in the ether could not 
have been much changed. After the extraction of the 
residue left after the evaporation of the absolute alcohol by 
ether and chloroform, some was still left untouched by these 
solvents ; on treatment of this residue with nitric acid the 
colour was discharged, the acid itself now becoming yellowish. 
That the above ethereal solution contained a slightly different 
colouring matter from that present in chloroform is proved by 
this experiment. An alcohol solution of clot was evaporated 
down, the residue extracted with chloroform, the latter eva- 
porated down ; it then left a brown amorphous pigment. On 
dissolving this in ether I obtained not sp. 10 but sp. 11; but 
when the residue from the alcohol solution already ex- 
tracted by chloroform was treated with ether, a reddish- 
