CHROM A.TOLOGY OF BLOOD OF SOME INVERTEBRATES. 487 
yellow solution was obtained which did give sp. 10 again, 
showing the splitting up of the echinochrome into two pig- 
ments. 
If a chloroformic solution obtained as above be evaporated 
and the residue 1 extracted with bisulphide of carbon, a red 
solution is obtained which gives sp. 12, Chart II. The residue 
is also soluble in benzene, giving similar bands. 
If the second of the methods mentioned above for obtaining 
echinochrome — namely, filtering off the clot from the “ serum,” 
drying on the paper, and extracting with absolute alcohol — be 
adopted a pale red solution is obtained, which gives the same 
spectrum as the alcohol solution of fresh clot, and the same 
spectrum with stannous chloride. 
An aqueous solution of dried echinochrome gave no 
distinct bands, but on adding stannous chloride the usual 
bands appear, which still persist after acidulating with hydro- 
chloric acid ; after which they read : first band, X 537 to 
X 513; second, X 505 to 484‘5 (?) ; the colour of the solution 
being reddish. 
An ether solution of the dried clot is reddish -yellow and 
gives sp. 13, II ; that this solution contains the same pigment 
as the alcohol solution is shown by adding stannous chloride 
which develops the bands referred to, measuring in this case : 
first band, X 533-5 to X 520; second, X 496-5 to X 477, the 
solution having a violet-red tint. On treatment of this with 
an ether solution of peroxide of hydrogen the bands produced 
by the stannous chloride were not changed. 
A chloroform solution of the dried clot is reddish yellow 
— and in one degree of dilution violet red — and gives sp. 14, 
Chart. II. On treating with stannous chloride the usual 
bands are seen : first, from X 540 to X 516 ; second, from X 505 
to X 484-5 (?). 
A bisulphide of carbon solution of the dried clot is 
violet red, and gives sp. 15, Chart II. The bands measure 
approximately, first X 566 to X 554"5, while the dark shading 
commences at X 537, and its darkest part ends at X 511-5, the 
1 Which is of a violet colour. 
