NOTES ON THE EMBRYOLOGY OF LIMULUS. 
521 
Notes on the Embryology of Limulus. 
By 
J. 8. Kingsley, D.Sc. 
Malden, Mass., U.S.A. 
With Plates XXXVII, XXXVIII, XXXIX. 
Several papers have been published upon the development 
of Limulus polyphemus, the titles of which will be found in 
the appended bibliography. Since Professor Ray Lankester 
has recently strongly advocated the Arachnidan affinities of 
Limulus it has seemed especially desirable to study the embry- 
ology of this form by means of sections, a method scarcely 
touched by Dr. Packard, the most voluminous writer upon this 
subject. 
I would here return my sincere thanks to my friend George 
H. Thompson for his assistance in obtaining material for my 
researches. 
I have not witnessed the process of oviposition, which, how- 
ever, has been described by Dr. Lockwood (' 70), 1 but with 
some of his conclusions I would express dissent. The eggs are 
not all laid above high-water mark, for I have found them half 
way between tides, and have frequently taken the male and 
female coupled together, and buried in the mud below low- 
water mark. 
All of my attempts at artificial impregnation have been 
unsuccessful, for animals kept in confinement, even when 
1 I have, in referring to the bibliography, adopted the admirable plan of 
Dr. E. L. Mark, by which the reference number gives the reader at once the 
approximate date of the article. A bibliography is appended. 
