Annals of the Transvaal Museum. 
51 
show a loose structure and are irregularly shaped. The nuclei of the 
second phase, that is of the second generation or gamagonous, take the 
nuclear stains more deeply ; they undergo reductions and accordingly 
appear smaller. Before the segmentation of the nuclei has taken place, 
they are oval-shaped. One is struck by the fact that the extracellular 
segmenting forms of the gamogonous generation produce parasites in far 
smaller numbers (fig. 3) than those found in the cells themselves (fig. 
4 — 55). I have not been able to give an explanation of this observation. 
In the blood no multiplication has as yet been seen, but my studies 
on these parasites are not yet concluded. Plasma granules were 
found in the blood, but this fact by no means necessitates a change 
of the above technology. It may also be possible that two 
agamogonous stages have to be distinguished, and the parasites 
which develop in the red corpuscles would have to be considered 
as the gamogonous generation. I am, however, of the opinion that the 
two stages in the cycle which I have called provisionally agamogony and 
gamogony, and which finally lead to the formation of the forms seen in 
the blood, succeed each other within the cell. Accordingly it is clear 
that the number of parasites increase as the disease develops. The forms 
which result from the segmentation of the last generation differ morpho- 
logically in no way from the parasites seen in the blood at the commence- 
ment of an infection. They possess, in proportion to the whole parasite, a 
large nucleus which contains a distinctive karyosome. In using Giemsa’s 
stain one can see differences due to the staining in the plasma of the bodies 
belonging to the agamogonous stage as well as of the gamogonous one. 
There are dark blue forms along with light blue ones containing larger 
alveli. It is possible that from the very start we have to deal with a 
sexual differentiation. The above nuclear phenomen can easily be studied 
in preparations stained according to the dry and moist methods of Giemsa, 
as well as in those stained with haemotoxylin. Microscopic examination 
of the various organs, particularly of the spleen, does not allow of definite 
conclusions. These organs represent a filter which retains the broken 
down parasites usually encountered in all protozoic diseases. When using 
the vital method I was never able to recognize the blue bodies, so that it is 
impossible for me to believe that these represent an assimulation product 
of the nuclei. The presence of Koch’s bodies in the kidneys is of second- 
ary importance. In East Coast Fever experimentally produced, either 
through the implantation of organs or in cases brought on by ticks, which 
finish rapidly with death, the formation of infarcts in the kidneys may 
not take place at all. My drawings of, *and the communication referring 
to Koch’s granules, concern fresh material obtained with a syringe from 
the lymphatic glands and the spleen. In cases of East Coast fever, where 
fever is present without parasites being present in the blood, the diagnosis 
can be made by examining juice from the spleen and lymphatic glands. 
If in the juice obtained from the puncture of the lymphatic glands or the 
spleen are seen Koch’s bodies containing only larger nuclei of a loose 
structure, and no forms of the second or gamogonous generation, it is 
possible that this latter does not take place at all, and no parasites are then 
found in the blood. Whether ticks which have been sucking the blood of 
such animals transmit the disease will be shown by later experiments. 
It remains to explain the fact that the blood of animals suffering from 
East Coast fever injected into healthy animals does not produce the 
disease. It is possible that the blood contains forms which can only 
develop in the tick, and which, injected into the animal, die. In the 
