20 THE QUESTION OF THE RELATION OF 
laid. I saw a tsetse fly settle on the cane, gradually walk 
along to the part where the pith was exposed, insert his pro- 
boscis and feed. After some little time I made an attempt to 
catch this fly, but unfortunately failed to do so. Of the accuracy 
of my observation in each case I have not the smallest doubt, 
and have referred to these two instances on many occasions 
in my writings on this and kindred subjects.’ 
Confirmation of these observations would be very interest- 
ing. It would explain why it is that tsetse flies have been 
found by many observers in areas where animal life was 
apparently quite absent. At present Mr. Maugham’s obser- 
vations stand alone. (See 4 Sleeping Sickness Bulletin,’ Vol. 3, 
No. 28, p. 271.) 
It will be seen from the above survey of the recent discoveries 
which have been made with regard to the relation of game 
animals to the trypanosomiases that there is at present not 
sufficient scientific evidence to justify the extermination of 
game as a means of clearing a district of diseases transmitted 
by blood-sucking insects. Evidence on the subject is difficult 
to collect, and often most untrustworthy. Microscopical 
examinations of blood are not usually conclusive, because the 
trypanosomes are frequently so scanty in the blood of an 
infected animal that the prospects of discovering one in the 
minute field of the microscope are extremely small. The 
method which appears to give the most reliable results is the 
injection of blood from the suspected animal into an animal 
which is known to be susceptible to the species of trypanosome 
about which information is required. 
In the controversy of ‘ Game versus Disease,’ what is so 
urgently needed is a very extensive series of inoculations, 
carried out in different districts on as large a scale as possible. 
And it is of the greatest importance that the susceptible animals 
should be inoculated not only with the blood of game animals, 
but also with that of all other animals, both wild and domestic, 
in the infected areas. 
Only by such experiments can it be definitely proved 
whether or not the game acts as a reservoir for the virus of 
the different trypanosome diseases, and whether it is the only 
reservoir. And it is these inoculation experiments that 
