GAME ANIMALS TO DISEASE IN AFRICA 21 
the friends of game preservation and true scientists should 
call for and insist upon. 
From the point of view of game preservation there are 
six questions which need answers, and until these questions 
are answered it is impossible to decide upon a definite and 
practically useful plan of campaign. Briefly these six questions 
are : — 
1. Are game animals the only wild animals which are 
acting as ‘ reservoirs ’ for trypanosomes ? 
2. Are the trypanosomes found in the blood of game 
animals pathogenic for man and domestic animals ? 
And if so, are not the trypanosomes found in the blood 
of other animals also pathogenic ? 
B. Are tsetse flies the only transmitting agents of these 
trypanosomes in the infected areas ? 
4. Are game animals the only source from which the tsetse 
flies or other transmitting agents draw their blood 
supply ? And if not, what are the other sources 
of supply ? 
5. Can tsetse flies live and breed upon food other than 
blood, such as plant juices ? 
6. Are the distribution, increase and spread of tsetse flies, 
if this latter occurs, dependent upon game alone ? And 
if not, what are the governing factors ? 
The first two of these questions can only be answered 
definitely by carrying out an extensive series of inoculation 
experiments, and it is essential that the susceptible animals 
should be inoculated not only with the blood of game animals, 
but also with the blood of all other animals and reptiles in the 
infected areas. 
The third question suggests its own necessary experiments. 
The fourth question is more difficult, but will be answered 
to some extent by the inoculation experiments and by the 
discovery of the pathogenic trypanosomes in the blood of 
other animals. 
For the fifth question I should like to suggest some such 
experiment as the following : — 
That a freshly killed bird or small mammal should be 
quickly skinned and the skin filled with honey or crushed banana 
