22 
Nitrogen content of globulin from cottonseed meal and its bacterial decomposition products. 
Per cent of original 
material. 
Per cent of total N. 
Per cent of 
groups de- 
composed. 
Per cent of 
organic N 
after bacte- 
rial action. 
Before. 
After. 
Before. 
After. 
Ammonia N 
4.55 
1.04 
1.87 
8.92 
27.77 
6.35 
11.41 
54.46 
Amid N 
1.80 
3.77 
10. 81 ' 
10.99 
23.02 
65.99 
42.22 
50.39 
17.48 
8.81 
Basic N 
15.80 
Nonbasic N 
75.40 
The nitrogen of 'the original material was composed of 10.99 
per cent amid, 23.02 per cent basic, and 65.99 per cent nonbasic 
compounds. Pure globulin from cotton seed, on the other hand, 
contains 10.3 per cent amid, 30.6 per cent basic, and 59.1 per cent 
nonbasic nitrogen. It is possible that the low percentage of basic 
nitrogen and the correspondingly high percentage of nonbasic nitrogen 
found above were due to incomplete hydrolysis, as Osborne has 
pointed out that some vegetable proteins require continuous boiling 
for 24 hours for complete hydrolysis. 
The above data show that 27.77 per cent of the nitrogen was 
converted into ammonia, and that 42.22 per cent of the amid, 50.39 
per cent of the basic, and 17.48 per cent of the nonbasic nitrogen 
compounds were decomposed. The organic nitrogen remaining 
after bacterial action was composed of 8.81 per cent amid, 15.80 
per cent basic, and 75.40 per cent nonbasic compounds. Comparing 
the above data with that obtained with the use of cottonseed meal, 
it is of special interest to note that globulin, when separated from 
the other nitrogen and nonnitrogenous constituents of cottonseed 
meal, undergoes bacterial decomposition in very much the same 
way as do the nitrogen compounds of cottonseed meal as a whole. 
In each instance the basic diamino acids were decomposed more 
rapidly than the other groups. The amids, however, were decom- 
posed more rapidly in the globulin than in cottonseed meal. 
ZEIN FROM MAIZE. 
With the exception of linseed meal the basic diamino acids of the 
preceding materials were decomposed more rapidly than the amids or 
monamino acids. The basic nitrogen in these materials varied from 
9.18 per cent to 23.02 per cent of the total nitrogen. In order to study 
the decomposition of a substance containing still less diamino nitrogen, 
zein was prepared from maize by alcoholic extraction. The product 
was not highly purified but the analysis shows that practically all 
the nitrogen was in the form of zein. 
