21 
to the materials reported above, that the nonbasic monamino acids 
of linseed meal were decomposed more rapidly than the nitrogen 
compounds of other groups. 
COCONUT MEAL. 
Preliminary ammonification experiments with this material indi- 
cated that the nitrogen constituents would be decomposed more 
slowly than in the materials reported above. After incubating 
one week practically no ammonia was found. Consequently the 
decomposition was allowed to take place for 12 days, but even then 
only a small amount of ammonia was formed. 
Nitrogen content of coconut meal and its bacterial decomposition products. 
Per cent of original 
material. 
Per cent of total N. 
Per cent of 
groups de- 
composed. 
Per cent of 
organic N 
after bacte- 
rial action. 
Before. 
After. 
Before. 
After. 
0.24 
.37 
.41 
2.28 
7.27 
11.21 
12. 43 
69.09 
Amid N 
0.38 
.52 
2.40 
11.52 
15.76 
72.72 
2.67 
21.15 
5.00 
12.09 
Basic N 
13.39 
Nonbasic N 
74.51 
It would seem that the carbohydrates and fats protected the 
nitrogen bodies from bacterial decomposition, since only 7.27 per 
cent of the total nitrogen was found as ammonia, and the absolute 
amounts of nitrogen in the different groups were only slightly 
different from those in the original material. But the magnitude 
of the experimental error was relatively too great to justify positive 
conclusions. 
The data show, however, that a higher percentage of the ammonia 
was derived from the basic nitrogen group than in any of the previous 
experiments. 
GLOBULIN FROM COTTONSEED MEAL. 
The globulin was prepared from cottonseed meal by extraction 
with a 10 per cent solution of sodium chlorid, then precipitated by 
saturating the solution with ammonium sulphate, redissolved in 
sodium chlorid solution, and dialyzed. The product was washed 
with alcohol and ether, and dried in vacuum over sulphuric acid, 
but was still impure as shown by the nitrogen content. Ammonifi- 
cation continued for three days. 
