18 
have already been given. Coconut meal, containing 3.30 per cent 
nitrogen, globulin from cottonseed meal, containing 16.38 per cent 
nitrogen, and zein from maize, containing 14.03 per cent nitrogen, 
were also used. Neither the globulin nor the zein was entirely pure, 
as is shown by the nitrogen content. 
casein. 
Nitrogen content of casein and its bacterial decomposition products. 
Per cent of original 
material. 
Per cent of total N. 
Per cent of 
groups de- 
composed. 
Per cent of 
organic N 
Before. 
After. 
Before. 
After. 
after bacte- 
rial action. 
3.93 
1.10 
0.95 
6.31 
31.96 
8.88 
7.67 
50.93 
Amid N 
1.54 
2.12 
8.87 
12.43 
17.11 
71.59 
28.57 
59.19 
28.86 
13 16 
Basic N 
11 34 
75 48 
The casein used was not pure. In addition to considerable ether 
soluble matter it probably contained small amounts of nitrogen 
bodies other than casein. Pure casein from cow's milk contains 
10.3 per cent of the nitrogen in the form of amids and 22.4 per cent 
as basic nitrogen compounds, while the above material yielded 12.43 
per cent as amids and only 17.11 per cent as basic compounds. 
The bacterial action was allowed to continue for three days, during 
which time 31.96 per cent of the total nitrogen was converted into 
ammonia. The amid nitrogen was reduced from 12.43 per cent to 
8.88 per cent of the total, basic nitrogen from 17.11 per cent to 7.67 
per cent, and nonbasic nitrogen from 71.59 per cent to 50.93 per cent. 
Expressed in percentages of decrease we find that 28.57 per cent of the 
amid nitrogen, 55.19 per cent of the basic, and 28.86 percent of the 
nonbasic nitrogen were ammonified. The organic nitrogen remain- 
ing at the close of the experiment was composed of 13.16 per cent 
amid, 11.34 per cent basic, and 75.48 per cent nonbasic nitrogen 
compounds. Comparing these percentages with the composition of 
the original casein it will be seen that the basic nitrogen compounds 
were decomposed more rapidly than the amids or nonbasic nitrogen 
compounds. 
DRIED BLOOD. 
Nitrogen content of dried blood and its bacterial decomposition products. 
Per cent of original 
material. 
Per cent of total N. 
Per cent of 
groups de- 
composed. 
Per cent of 
organic N 
after bacte- 
rial action. 
Before. 
After. 
Before. 
After. 
6.11 
.67 
1.09 
5.65 
45.19 
4.95 
8.06 
41.76 
Amid N 
1.23 
2.52 
9.77 
9.09 
18.64 
72.26 
45.53 
56.75 
42.17 
9.04 
Basic N 
14.71 
Nonbasic N 
76.25 
