2 HUMANE TREATMENT OF ANIMALS USED IN RESEARCH 
States Government, or by or through any person or agency pursuant to contract 
or authorization of the United States Government, to any person who uses live 
animals in research, experiments, tests or training unless the person applying 
for or receiving the grant has a certificate of compliance with this Act, issued by 
the Secretary of Health, Education, and Welfare. 
Sec. 3. The Secretary shall, pursuant to such rules and regulations as he may 
prescribe, issue certificates of compliance to persons applying therefor upon proof 
satisfactory to him — 
(a) that the applicant’s proposed methods and procedures involving the 
use of live animals are in accordance with the requirements of this Act and 
the policy of the Congress ; 
(b) that the applicant’s personnel and facilities are adequate and ap- 
propriate to enable it to comply with the requirements of this Act and the 
policy of the Congress stated herein ; and 
(c) that the applicant has complied or is equipped to comply with the re- 
quirements of section 4 of this Act. 
Sec. 4. Each person to whom a certificate of compliance has been issued, and 
each agency or instrumentality of the United States which uses live animals for 
research, experiments, tests or training shall comply with the following re- 
quirements : 
(a) All premises where animals are kept shall provide a comfortable resting 
place, adequate space and facilities for normal exercise, and adequate sanitation, 
lighting, temperature control and ventilation ; 
(b) Animals shall receive adequate food and water and shall not be caused to 
suffer unnecessary or avoidable pain through neglect or mishandling; 
(c) Animals used in any experiment which would result in pain shall be anes- 
thetized so as to prevent the animals feeling the pain during and after the ex- 
periment except to the extent that the use of anestetics would frustrate the ob- 
ject of the experiment, and in any event, animals which are suffering severe 
and prolonged pain shall be painlessly killed. Unless the project plan on file 
with the Secretary specifies a longer period during which animals must be 
kept alive for essential purposes of the experiment or test, consistent with this 
Act and the rules and regulations hereunder, animals which are seriously injured 
as a result of the experiment shall be painlessly killed immediately upon the 
conclusion of the operation inflicting the injury ; 
(d) An accurate record shall be maintained of all experiments and tests per- 
formed. Procedures shall be employed to make possible the identification of 
animals subjected to specified experiments and tests, and a record shall be kept 
of the disposition of such animals ; 
(e) AH cages or enclosures containing animals shall be identified by cards 
stating the nature of the experiment or test, or numbers which correspond to 
such a description in a record book ; 
(f) Painful experiments or tests on living animals shall be conducted only 
by persons licensed under section 5 of this Act or by students in an established 
training institution who are under the direct supervision of a licensee and all 
animals used by the students in practice surgery or other painful procedures 
shall be under complete anesthesia and shall be killed without being allowed 
to recover consciousness ; 
(g) No experiment or test on living animals shall be undertaken or performed 
unless a project-plan is on file in such form as the Secretary may prescribe, de- 
scribing the nature and purposes of the project and the procedures to be em- 
ployed with respect to living animals ; 
(h) An annual report and such additional reports or information as the 
Secretary may require by regulation or individual request shall be submitted to 
the Secretary. The annual report shall specify the number of animals used, 
the procedures employed, and such other matters as the Secretary may prescribe, 
and shall include a copy of any published work prepared or sponsored by the 
reporting person or agency, involving the use of live animals ; and 
(i) Authorized representatives of the Secretary shall be given access to the 
animals and to the premises and books and records of the agency or person 
for the purpose of obtaining information relating to the administration of this 
Act, and such representatives shall be authorized to destroy or require the de- 
struction of animals in accordance with rules, regulations, or instructions issued 
by the Secretary, in conformance of this Act. 
