Cocaine B/AP-1 
Cocaine B 
Saline B 
Cocaine A/AP-2 
Cocaine A/AP-1 
Cocaine A 
Saline A 
FIGURE 6. P 32 - end-labeled AP- 1 oligonucleotide binding of striatal extracts 
(12 pg) prepared 2 hours after IP injection of saline (lanes 1 and 
5) or cocaine , 30 mg/kg (lanes 2, 3, 4, 6, and 7) in two sets of 
adult animals (A and B). The arrow designates the position of the 
specific band induced by cocaine administration . Basal levels of 
AP-1 DNA binding activity (lanes 1 and 5) were induced 3- to 3.5- 
fold by cocaine (lanes 2 and 6). The specificity of binding is 
demonstrated by competition with cold AP-1 oligonucleotide 
(lanes 3 and 7), but not by cold AP-2 oligonucleotide (lane 4), 
both used at 65-fold excess over labeled probe. 
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