800 
Cerebellum 
FIGURE 1 2. The increase in c-fos mRNA is independent of protein synthesis. 
The bars represent results expressed as counts per minute 
(cpm) of protected c-fos mRNA from an RNase protection 
assay. Rats were treated with saline (sal); cocaine 30 mg/kg 
IP (coc); or anisomycin 100 mg/kg given 20 minutes prior to 
cocaine 30 mg/kg IP (coc+aniso). Anisomycin pretreatment 
did not prevent the increase in c-fos mRNA induced by cocaine. 
Anisomycin alone also stimulated an accumulation of c-fos 
mRNA (not shown). In fact, the increase produced by 
anisomycin alone was additive with that produced by cocaine. 
At the 30 and 40 mg/kg doses of cocaine, an increase in c-fos 
mRNA occurred in cerebellum. The cerebellar increase also 
was not attenuated by anisomycin. 
198 
