Fig. 4; Schei 
cell therapy. 
A piece of tail from a mouse homozygous for the recvjiiu.i^awi. 
activating gene 2 (Rag2) mutation was removed an 
v>'^ r*"i Af' ^ 
» : ^ ^aCi. W - - - ^ ^ 
fibroblast-Iike ceils grew’ out, they were used as donors for nuciem 
transfer by direct Injection into enucleated Mil oocytes using a 
Piezoelectric driven micromanipulator. EmJDryonic stem (ES) cells 
isolated from the NT-derived blastocysts were genetically repaired 
by homologous recombinatiGn. After repair, the ntES cells were 
differentiated in vitro into embr/oid bodies (EBs), infected with the 
HoxB4iGFP retrovirus, expanded, and injected into the tail vein of 
irradiated, Rag2-deficient mice (after (Rideout et al., 2002)). 
Fig 4 Correction of a Genetic Defect by Combination of 
Therapeutic Cloning and Gene Therapy 
HAxMCFf yMtar 
jf '9 
K 
$. Repsair R»g2 g«fM 
«■ ES »V hoMiog. 
r*e«iiditeaU«n 
\ 
// 
3. Nuel«9r tramfar kni* 
X lUitfvaU anil eulti»« 
to «l«A«d U»%to«y«t 
4. KotiU Ko««nto 
Rafi EB 
PRE-PXJBLICATION VERSION 
