76 
Psyche 
[Vol. 92 
Col 
ft 
Day of Experiment 
Total 
Days 
1 2 5 11 13 18 29 76 
1 
25 
5 
• 
1 
8 
• 
1 
11 
— ■ 
2 
14 
1C 
9 
AO 
ID 
i 7 
oa 
OQ 
1 / 
18 
— 
2 
19 
• 
1 
50 
go 
12 
34 
C7 
(O 
OQ 
Of 
n/Day 
= 
68656772 
Fig. 1 . Occurrence and apparent duration of spontaneous polydomy for all colo- 
nies which budded during the course of the experiment. Indicated are colony 
numbers (Col #), day of experiment on which polydomy was observed (Day of 
Experiment), number of colonies which were polydomous on each observation day 
(n/Day), and total number of days over which each colony appeared to remain 
polydomous (Total Days). 
size (as indicated by the number of workers) appeared to have a 
significant influence on polydomy (see Figure 2). Budding occurred 
in 1 1 of 26 colonies (42.3%) which contained 60 or more workers, 
but in only 1 of 31 colonies (3.2%) with less than 60 workers (x 2 = 
9.40, df = 1 , P < 0.0 1 ). Overall, colonies which budded were signifi- 
cantly larger than those which failed to do so (t = 3.554, df = 55, P< 
0.001). This relationship held even if colonies which were queenless 
when collected (n = 14) were omitted from the analysis (t = 3.268, 
df =41, P< 0.01); or if colonies which were either queenless (n = 14) 
or polygynous (n = 6) when collected were omitted (t = 2.134, df = 
35, P < 0.05). Among colonies with 60 or more workers there was 
no significant difference in size between colonies which budded and 
those which did not (t = 1 .305, df = 24, P > 0.05). 
