394 
Psyche 
[Vol. 92 
Acroneuria carolinensis has been recorded from 1 1 states and 
three Canadian provinces. It ranges from Manitoba, Ontario and 
Quebec in southern Canada to Tennessee and South Carolina in the 
United States (Peckarsky, 1979). 
Materials and Methods 
Panther Creek originates in Nicholas County, 3 Vi miles (5.6 km) 
southeast of the Greenbrier County line, near Saxman, West Virginia 
at an elevation of 3000 feet (914 m). Panther Creek flows northward, 
emptying into the Gauley River at an elevation of 820 feet (250 m); it 
has a total fall of 1180 feet (360 m) and a total length of 9.6 miles 
(15.4 km). The drainage basin area is 16.88 square miles (43.7 m 2 ) 
(Reger, 1921). 
The study area is located along Route 39, 13.7 miles (22.1 km) 
west of the junction of Routes 19 and 39 at Summersville, at an 
elevation of 2200 feet (671 m). Collections were made in a riffle area 
161 feet (49 m) below a concrete dam. The riffle was 36 feet (11m) 
long and had an average width of 25.6 feet (7.8 m). Substrate con- 
sists of rocks with an approximate size range of 6 to 18 inches (15 to 
46 cm) in diameter. 
Qualitative samples were collected monthly from December 1984 
to November 1985. After disturbing the substrate, a fine meshed 
dredge (mesh size: 4 mm, December-May; 1 mm, June-November) 
was held immediately below the disturbed area to collect the drifting 
naiads. Contents of the dredge were emptied into a white enamel 
pan to facilitate sorting. Naiads were immediately killed and pre- 
served in 70 percent ethyl alcohol. 
Temperature of the stream was recorded monthly using a Fisher 
instantaneous thermometer. During the emergence period, tem- 
perature was recorded weekly. 
Water chemistry was measured monthly using a Hach chemical 
kit (Model AL-36B). Dissolved oxygen, carbon dioxide, hardness, 
alkalinity and pH were measured in the field. All variables were 
measured in mg/1 except for pH. 
Total length (excluding antennae and cerci) was measured by 
placing the naiad on a plastic millimeter ruler and viewing under a 
binocular dissecting scope. Naiads were measured to the nearest 0.5 
mm and placed in 1 mm class intervals. Length-frequency histo- 
