1986] Kane & Brunner — Neaphaenops tellkampfi 235 
Methods 
Electrophoretic data gathered from a total of 1 8 populations (Fig. 
1) of Neaphaenops tellkampfi were analyzed in this study. All of the 
electrophoretic data for ten of these populations were gathered dur- 
ing the course of the present study, between 1980 and 1983. These 
ten populations include three each of N. t. henroti (BL, CW and 
T/SS; Fig. 1), N. t. meridionals (H, OS and ST; Fig. 1) and N. t. 
viator (C, CB and S; Fig. 1) as recognized by Barr (1979b). The 
tenth population (F; Fig. 1) is a purported meridionals X tellkampfi 
hybrid on morphological grounds (Barr, 1979b). Most, but not all, 
of the electrophoretic data on the eight populations of N. t. tell- 
kampfi (B, BH, GO, HA, LB, P, RB and WH; Fig. 1) were col- 
lected in 1977-78 and reported by Turanchik and Kane (1979). 
Modifications of and additions to the nominate tellkampfi data set 
will be discussed in appropriate sections below. All 18 of the popula- 
tions sampled, with the exception of the SS and T sites of henroti, 
represent a single cave location. During the course of the study 
permission to sample the SS site was rescinded before a sample 
adequate for complete electrophoretic survey could be obtained. 
Subsequently the nearby T site was located but it harbored a much 
smaller henroti population and failed to yield a large enough sample 
to obtain data on all electrophoretic loci. Pooling of the data from 
the two sites, which appears to be justified by their geographic 
proximity, did produce a complete set of electrophoretic data. 
Beetles were maintained alive at 5°C or frozen at -80° C prior to 
electrophoresis. All electrophoresis was conducted on vertical 
polyacrylamide slab gels using an Ortec Model 4200 Electrophoresis 
System or a Hoefer Scientific SE600 System. Sample preparation 
and run procedures used in this study were similar to those dis- 
cussed by Giuseffi et al. (1978) and Turanchik and Kane (1979). 
Each animal provided enough homogenate for two assays. 
Six enzyme systems provided a total of seven consistently scora- 
ble loci. These included: alkaline phosphatase (ALP) (1); esterase 
(EST) (1); malate dehydrogenase (MDH) (2); phosphoglucomutase 
(PGM) (1); phosphoglucose isomerase (PGI) (1); and, xanthine 
dehydrogenase (XDH) (1). In addition a general protein (GP) stain 
revealed two sets of consistently scorable bands which are also 
