1986] 
Kane & Brunner — Neaphaenops tellkampfi 
243 
Table 5. Hierarchichal F-statistics and heterogeneity chi-square analyis of allelic 
frequencies between subspecies of Neaphaenops tellkampfi 
Locus 
f ct 
Fcs 
Fst 
X 2 
ALP 
0.022 
0.023 
-0.001 
16.224** 
EST 
0.958 
0.000 
0.958 
1564.120*** 
GPT-1 
0.081 
0.074 
0.007 
22.227** 
MDH-2 
0.044 
0.035 
0.009 
17.900** 
PGI 
0.988 
0.000 
0.988 
1841.171*** 
TOTAL 
0.546 
0.038 
0.528 
** = PC0.01; *** = P <0.005 
F C t — correlation of random gametes in local populations relative to the gametes of 
the total population 
F cs = average correlation over subspecies of uniting gametes relative to those of 
their own subspecies 
F st = correlation of random gametes within subspecies relative to gametes of the 
total population 
subspecies N. t. tellkampfi. Thus, an analysis of gene flow in all four 
N. tellkampfi subspecies is appropriate since both H. subterraneus 
and P. hirtus are sympatric with N. tellkampfi. Further, the range of 
H. subterraneus examined by Caccone (1985) is more comparable to 
that of N. tellkampfi (s.l.) than simply to that of nominate 
tellkampfi. 
The Slatkin analysis suggests that N. tellkampfi may be qualita- 
tively described as a species in which gene flow level is low. Alleles 
with low incidence values (i/d) have high conditional frequencies (p) 
(Fig. 3). Caccone (1985) showed that P. hirtus is also a species with 
low gene flow levels. By contrast, PI. subterraneus is seen to be a 
species with intermediate gene flow levels (Caccone, 1985). As indi- 
cated earlier, the range of H. subterraneus is larger than and 
includes the entire range of N. tellkampfi. Unlike N. tellkampfi and 
P. hirtus, however, H. subterraneus is troglophilic (facultative cave 
dweller) and thus is capable of some dispersal on the surface in 
addition to the subterranean routes available to troglobites. Analy- 
sis of the eight nominate tellkampfi populations indicates a high 
level of gene flow within this subspecies (Fig. 3) despite some het- 
erogeneity in gene frequencies among these populations (Table 4). 
The overall pattern of gene flow is generally consistent with the 
pattern of genetic differentiation obtained from the F-statistics. 
