1964] 
Blum , Moser , Cordero — Chemical Releasers 
7 
then the deposited compounds could be expected to be quite non- 
volatile. That this is the case is indicated by the fact that artificial 
trails prepared from the poison glands (and vesicle) of Trachymyr- 
mex were highly active when tested three weeks after their prepara- 
tion. Furthermore, when poison glands of Atta cephalotes were 
crushed on microscope slides, the semisolidified secretion retained its 
original appearance for 28 days at room temperature (approximately 
28°C.) and released strong trail following behavior in Trachymyr- 
mex when artificial trails were prepared from chloroform extracts. 
SUMMARY 
In Cyphomyrmex rimosus (Spinola), Brachymyrmex septentrionalis 
(McCook), A cromyrmex octospinosus (Reich) and Atta cephalotes 
(Linnaeus), four attine genera representing the broad phylogenetic 
development of the tribe Attini, odor trail substances originate in the 
poison glands. These substances, when cross genera tested, were 
found to be non-generic specific. This fact is interpreted as further 
evidence for the close relationship of the attine genera. 
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