1962 ] 
Ghent and Gary — Alarm Releaser 
5 
6o° C., had the characteristic bee sting scent. Some of this scent was 
retained by the yellow residue. 
Vapor phase chromatograms of the first fraction showed evidence 
of only the solvent, methylene chloride. In both of the last two frac- 
tions only two components in addition to the solvent were detected: 
water and the scented substance presumed to be the alarm releaser. 
Attempts to concentrate the odoriferous component by evaporation of 
the methylene chloride were only partially successful. Vapor phase 
chromatograms indicated that, although it was slightly concentrated 
during evaporation, the greater part was lost. 
60 
• distillate 
o solvent 
to _ _ 
uj 50 
H 
3 
Z 
2 
t^40 
£30 
oc 
I— 
u20 
UJ 
03 
6 
10 - 
t COmm 
100 
10 
50 
DISTILLATE l.) 
Figure 2. Attraction of worker bees to distilled extract of bee stings. The 
distillate was pipetted onto filter paper and introduced in screen cages laid 
over the combs of the colony. Bees attracted to each cage were collected and 
counted. 
The effect of the whole distillate on bee behavior was tested by the 
same method as that used for the crude extract. Data on attraction 
