194 
Psyche 
[Vol. 95 
An anamalous Gpi (glucose phosphate isomerase, EC 5. 3. 1.9) and 
Ao (aldehyde oxidase, EC 1.2.3. 1) banding pattern was present in 
low frequency among V. pergandei queens (buffer I, 25 min at 250 
V). Since we were unable to verify Mendelian inheritance of the 
variant pattern, which could not be interpreted easily in terms of 
known enzyme quaternary structure, we have omitted these loci 
from the analysis. The monomorphic loci resolved from V. pergan- 
dei were (enzyme trivial name, E.C. code and best running buffer 
are listed after each): Aat-1 (aspartate aminotransferase, EC 2.6. 1.1; 
I); Ac (aconitase, EC 4.2. 1.3; C); Dia (diaphorase, EC 1.6.*.*; D); 
G3p (glycerol-3-phosphate dehydrogenase, EC 1.1. 1.8; A); G6pdh 
(glucose-6-phosphate dehydrogenase, EC 1.1.1.49; A) Hk (hexose 
kinase, EC 2.7. 1.1; I) Lap (leucine aminopeptidase, EC 3.4.11.1; I); 
Ldh (lactate dehydrogenase, EC 1.1.1.27; D); Mdh-2 (C); Pep (pep- 
tidase, phenylalanyl-prolyl substrate; C); Pgd (6-phosphogluconate 
dehydrogenase, EC 1.1.1.44; D). 
We resolved allozyme products of 30 presumptive genetic loci 
from A. versicolor queens. Only Pgm was polymorphic with 2 
alleles designated F (fast migrating) and S (slow). Best resolution 
was given by buffer D, run for 30 min at 250 V. The monomorphic 
loci were (enzyme trivial name and EC code [if not listed above] and 
best buffer are listed after each): Aat-1 and Aat-2 (D); ^4c (D); Ada 
(adenosine deaminase, EC 3. 5.4.4; D); Ak (Adenylate kinase, EC 
2.7.1.20; A); A /d (aldolase, EC 4.1.2.13; D); Ao (D); Apk (arginine 
phosphokinase, EC 2.7. 3. 3; D); Dia (I); Est (D); Fum (fumarase, EC 
4.2. 1.2; D); Gapdh (glyceraldehyde phosphate dehydrogenase, EC 
1.2.1.12; C); Gldh (glucose dehydrogenase, EC 1.1.1.47; A); Gpi 
(C); G3p (A); G6pdh (B); Hbdh (hydroxybutyrate dehydrogenase, 
EC 1.1.1.30; D); Hk (C); Idh-l and Idh-2 (C); Lap (C); Ldh (B); 
Mdh-l and Mdh-2 (C); Me (malic enzyme, EC 1.1.1.40; A); Pep 
(glycyl-leucyl substrate; C); Pgd (A); Sod (superoxide dismutase, 
EC 1.15.1.1; D); Sordh (sorbitol dehydrogenase, EC 1.1.1.14; I). 
Statistical analyses. The within group relatedness for foundress 
associations was calculated from the relationship: 
r = 2*F st /(l + Fit), 
where F st and Fit are Wright’s inbreeding coefficients (Hamilton 
1972, Pamilo 1984, McCauley et al. 1988). The F st and F it were 
estimated for each polymorphic locus using Long’s (1986) proce- 
