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Psyche 
[Vol. 95 
ethanol and does not affect the condition of the ovaries by dehydra- 
tion. The ovaries were prepared by removing the subgenital plate 
with a pair of tweezers under a binocular microscope at 30X magni- 
fication. Ovariole development, contents of the receptacle and pres- 
ence of corpora lutea were studied at 30X to 70X magnification. The 
condition of the ovaries was rated using the following classification, 
based on a system by Buschinger (1968a) and Buschinger and Allo- 
way (1978). 
Inseminated, fertile queens. Ovarioles are elon- 
gated, corpora lutea are present. In the reproduc- 
tive season they contain numerous eggs with white 
yolk deposits. 
Old, inseminated females, sterile. No corpora lutea 
present, ovarioles are short. In gynomorphic fe- 
males, wing muscles replaced by fat body. Fat 
body usually yellow. 
Young, inseminated, sterile females. No corpora 
lutea, ovarioles are short, sometimes they contain 
one or two eggs. In gynomorphic females, wing 
muscles not yet degenerated, fat body white. 
Inseminated, sterile, but eggs in development. 
Ovarioles more or less elongated, no corpora lutea 
present. 
Not inseminated, sterile. 
Not inseminated, ovarioles elongated, containing 
eggs. Corpora lutea present. 
A-females 
a-females 
b-females 
b->A-females 
c-females 
C-females 
Since the color of the fat body, which changes from white in 
younger females to yellowish in older ones, was not noted in all 
cases, we do not differentiate between freshly inseminated, sterile 
females (b) and those which have already been inseminated during a 
past season (a-females). Likewise, we do not differentiate between 
older uninseminated females and young uninseminated females, 
which have not yet started sexual behavior. As sexuals eclose in late 
June and July in most areas, inseminated females collected in June 
or July should all have mated already during the last years, and thus 
be a-females. Whereas all recently eclosed gynomorphic females are 
winged, it is not possible to tell this year’s intermorphic females 
from older ones without judging the color of the fat body. Only 
colonies with several A-females were called polygynous; those with 
