150 
Psyche 
[Vol. 91 
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Days after copulation 
Figure 4. The quantity of material remaining in the female’s bursa copulatrix as a 
function of the time since copulation. Line shows change in mean values for days 0 
through 7. 
and second matings separated by 24 hrs (Table 2). Several compari- 
sons are valuable here. First, there was no significant difference 
between the amount of material passed in first copulations in 1979 
and 1983 (t = 0.614, 26 df, 0=0.27). In addition, males passed 
significantly more material 24 hrs after the first mating than they did 
10 min after the first mating (t=2.95, 17 df, p= 0.004). These 
comparisons point out the consistency in the observations between 
the two years and more importantly that while copulation durations 
indicate that males have substantially recovered in 24 hr their supply 
is still somewhat depleted. However, this depletion may be a result 
of housing males between matings without food and water. In fact 
most of these males flew immediately to nectar sources upon release 
in the field. There is much to be learned about the performance of 
males in third matings and their performance in second matings 
when they have access to nectar. 
Use of secretions by females 
The rate at which females deplete the secretions they have 
received from males is greatest between the second and fifth days 
